Anti-SKAP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75563

Core Product Specifications and Parameters

Biological Background: SKAP2 Function and Localization

• Protein identity and isoforms: SKAP2, also known as Src kinase-associated phosphoprotein 2, is a 41 kDa cytoplasmic adaptor protein belonging to the SKAP family. It is also referred to as PRAP, RA70, SAPS, SCAP2, SKAP55R, or SKAP-HOM.
• Core function in immune cell adhesion: SKAP2 may be involved in B-cell and macrophage adhesion processes. In B-cells, it couples the B-cell receptor (BCR) to integrin activation, facilitating immune cell attachment and migration.
• Role in src signaling pathway: The protein participates in the src signaling pathway, acting as an adaptor to mediate downstream signals.
• Subcellular localization: SKAP2 is predominantly localized in the cytoplasm, where it interacts with signaling molecules.
• Tissue expression: It is ubiquitously expressed across tissues, with confirmed presence in platelets at the protein level.
• Structural features and post-translational modifications: SKAP2 contains an SH3 domain and is classified as a phosphoprotein. It has been identified in proteomics studies and its 3D structure is partially characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-120). Consider using this antibody to detect full-length SKAP2 and monitor potential N-terminal cleavage events.
• For western blotting, a band at approximately 41 kDa is expected. Validate the antibody in relevant B-cell or macrophage cell lines (e.g., Ramos, RAW264.7) to confirm specificity.
• In immunofluorescence/ICC, anticipate cytoplasmic staining. Co-staining with membrane or organelle markers can help assess localization under different activation conditions.
• Since the antibody is polyclonal, it may recognize multiple epitopes; verify in knockout or knockdown controls to ensure target specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutes from concept to clinic. For studies on B-cell adhesion and src signaling, we offer validated antibody pairs, optimized polyclonal antibodies like this SKAP2 rabbit pAb, and a broad portfolio of ancillary reagents. Our team also excels in rare target raw-material sourcing, ensuring you get the critical tools needed for your immunoassay development.