Anti-SIVA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O15304

Core Product Specifications and Parameters

Biological Background: SIVA1 Function and Localization

• SIVA1 encodes the apoptosis regulatory protein Siva, also known as CD27-binding protein (CD27BP), which plays a critical role in CD27-mediated apoptosis (UniProt: O15304).
• The protein inhibits the anti-apoptotic activity of BCL2L1 isoform Bcl-x(L), thereby promoting cell death pathways (UniProt: O15304).
• Siva suppresses NF-kappa-B activation and enhances T-cell receptor-mediated apoptosis, implicating it in immune regulation (UniProt: O15304).
• Multiple isoforms are produced by alternative splicing, including Siva-1 and Siva-2, which may have distinct functional properties (UniProt: O15304 keywords).
• Siva localizes to both the cytoplasm and nucleus, suggesting dual roles in cytoplasmic signaling and nuclear events (UniProt: O15304).
• The protein contains a zinc-binding domain and is classified as a metal-binding phosphoprotein, with potential involvement in host-virus interactions and pharmaceutical targeting (UniProt: O15304 keywords).
• SIVA1 is ubiquitously expressed, with higher levels in thymus, testis, ovary, prostate, small intestine, and spleen, and lower levels in colon (UniProt: O15304).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-110, which includes the N-terminal region; this region is known to contain functional domains important for apoptosis induction and protein interactions. For detection of full-length SIVA1 (~19 kDa), consider using whole-cell lysates from tissues with high expression such as thymus or testis.
• In Western blot (WB), the antibody detects a band around 19 kDa; however, due to alternative splicing, multiple isoforms (e.g., Siva-1 and Siva-2) may be present, leading to potential multiple bands. Validate in your relevant sample system.
• For immunohistochemistry (IHC-P), antigen retrieval methods may influence detection; consider using citrate buffer with heat-induced epitope retrieval and test on positive control tissues like spleen or thymus.
• In ELISA applications, the antibody can serve as a capture or detection reagent; pairing with another SIVA1 antibody may improve sensitivity. Validate assay conditions empirically.
• Cross-reactivity with human and mouse has been reported; for other species, perform sequence alignment to assess homology before experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials. For apoptosis and immune-oncology studies, CamelBio provides validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our end-to-end sourcing services, from concept to clinic, ensure reliable access to rare-target antibodies like anti-SIVA1, accelerating your assay development.