Anti-SIRT6 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - Q8N6T7

Core Product Specifications and Parameters

Biological Background: SIRT6 Function and Localization

• NAD-dependent protein deacylase and mono-ADP-ribosyltransferase that plays an essential role in DNA damage repair, telomere maintenance, metabolic homeostasis, inflammation, tumorigenesis and aging. Related references: PMID:18337721 PMID:19135889 PMID:19625767
• Histone deacetylase: catalyzes deacetylation of histone H3 at Lys-9, Lys-18, and Lys-56 (H3K9ac, H3K18ac, H3K56ac), suppressing target gene expression of transcription factors including NF-kappa-B. Related references: PMID:19625767 PMID:21362626 PMID:23892288
• Involved in DNA double-strand break (DSB) repair by sensing breaks, recruiting repair proteins, and deacetylating H3K9ac/H3K56ac. Also deacetylates non-histone proteins such as DDB2 and p53/TP53 to promote repair. Related references: PMID:23911928 PMID:31995034 PMID:32538779 PMID:29474172 PMID:32789493
• Maintains telomere integrity by deacetylating histone H3 in telomeric chromatin, regulating telomere position effect and movement after DNA damage. Related references: PMID:18337721 PMID:19625767 PMID:21847107
• Tumor suppressor: inhibits glycolysis (Warburg effect) via HIF1A corepression and deacetylation of PKM2; also regulates gluconeogenesis by deacetylating FOXO1 and KAT2A/GCN5. Related references: PMID:23217706 PMID:26787900 PMID:23142079 PMID:25009184
• Possesses defatty-acylase activity: mediates demyristoylation and depalmitoylation of target proteins (e.g., RRAS2, TNF), regulating their secretion. Related references: PMID:23552949 PMID:24052263 PMID:27322069 PMID:28406396
• Mono-ADP-ribosyltransferase activity modifies PARP1, TRIM28/KAP1, and SMARCC2/BAF170, contributing to DNA repair, cellular senescence, retrotransposon silencing, and transcriptional regulation. Related references: PMID:21680843 PMID:22753495 PMID:27322069 PMID:27568560
• Subcellularly localizes to the nucleus, chromosome, telomeres, and endoplasmic reticulum; post-translationally modified by acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein covering the full-length human SIRT6 (amino acids 1–355). This design may confer high specificity for native SIRT6 across assays.
• In Western blot, the predicted molecular weight is ~39 kDa (UniProt), with observed bands typically around 36–39 kDa. Validate detection in your experimental system and consider using appropriate loading controls.
• For immunoprecipitation (IP) and immunofluorescence (IF/ICC), we recommend titrating the antibody concentration and including proper positive/negative controls relevant to SIRT6 expression.
• For immunohistochemistry on paraffin sections (IHC-P), antigen retrieval optimization may be necessary. Test both heat-induced and enzymatic retrieval methods.
• Cross-reactivity with mouse and rat SIRT6 allows seamless translational studies, but confirm reactivity in your specific tissues or cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is committed to providing diagnostic manufacturers, laboratories, and research institutes with a streamlined, one-stop sourcing experience for IVD raw materials, technical services, and expert consulting—from concept to clinic. This anti-SIRT6 rabbit monoclonal antibody is a valuable tool for investigations into DNA repair, aging, metabolism, and oncology. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, and we specialize in sourcing rare-target raw materials to accelerate your diagnostic development.