Anti-SIRT6 Polyclonal Antibody for WB, IHC-P, ELISA - Q8N6T7

Core Product Specifications and Parameters

Biological Background: SIRT6 Function and Localization

• SIRT6, also known as NAD-dependent protein deacylase sirtuin-6 or hSIRT6, is a multifunctional enzyme possessing deacetylase, defatty-acylase, and mono-ADP-ribosyltransferase activities. Related references: PMID:18337721 PMID:19135889 PMID:19625767
• As a key histone deacetylase, SIRT6 removes acetyl groups from histone H3 at lysines 9, 18, and 56 (H3K9ac, H3K18ac, H3K56ac), thereby regulating transcription, DNA repair, and chromatin structure. Related references: PMID:19625767 PMID:21362626 PMID:23892288
• Essential for DNA double-strand break (DSB) repair, SIRT6 acts as a DSB sensor, recruiting repair proteins such as SMARCA5 and deacetylating histones and non-histone proteins like DDB2 and p53/TP53 to facilitate repair. Related references: PMID:23911928 PMID:31995034 PMID:32538779 PMID:29474172 PMID:32789493
• SIRT6 maintains telomere integrity and pericentric heterochromatin silencing, protecting cells from genomic instability and cellular senescence. Related references: PMID:18337721 PMID:19625767 PMID:21847107 PMID:27043296
• SIRT6 is a master metabolic regulator, suppressing glycolysis by inhibiting HIF1A and deacetylating PKM2, and modulating gluconeogenesis and lipid metabolism via FOXO1 and KAT2A/GCN5. It also acts as a tumor suppressor by inhibiting the Warburg effect. Related references: PMID:24012758 PMID:26787900 PMID:23217706 PMID:23142079 PMID:25009184
• SIRT6 exhibits defatty-acylase activity, catalyzing demyristoylation and depalmitoylation of targets such as RRAS2 and TNF, which regulates protein secretion. Related references: PMID:23552949 PMID:24052263 PMID:27322069 PMID:28406396
• The mono-ADP-ribosyltransferase activity of SIRT6 modifies proteins like PARP1, TRIM28, and SMARCC2, contributing to DNA repair, cellular senescence, and silencing of LINE-1 retrotransposons. Related references: PMID:21680843 PMID:22753495 PMID:27322069 PMID:27568560
• Subcellular localization: SIRT6 is predominantly found in the nucleus, associated with chromosomes and telomeres, and also detected at the endoplasmic reticulum.

Experimental Guidance and Technical Tips

• For Western blotting, observed bands may appear at 36 kDa and 39 kDa, potentially reflecting isoforms or post-translational modifications; validate with appropriate controls and molecular weight markers.
• In IHC-P, consider optimizing antigen retrieval and detection conditions to achieve specific nuclear staining in tissue sections.
• ELISA can be used for quantitative analysis of SIRT6 levels; include standard curves and confirm specificity with relevant controls.
• As a polyclonal antibody, be aware of potential lot-to-lot variability; validate performance in your specific sample system and application.
• Cross-reactivity with mouse and rat SIRT6 allows for comparative studies across these species, but always verify reactivity in your model of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-SIRT6 antibody, bulk ancillary reagents, and sourcing of rare targets. Our comprehensive supply capabilities support your development of assays targeting SIRT6, a key regulator of aging, metabolism, and tumorigenesis. Contact us to streamline your procurement and accelerate your project from concept to clinic.