Anti-SIRT6 Monoclonal Antibody for WB, ELISA - Q8N6T7

Core Product Specifications and Parameters

Biological Background: SIRT6 Function and Localization

• SIRT6, also known as SIR2L6 or hSIRT6, is an NAD-dependent protein deacylase, deacetylase, and mono-ADP-ribosyltransferase that plays essential roles in DNA damage repair, telomere maintenance, metabolic homeostasis, inflammation, tumorigenesis, and aging (PubMed:18337721; PubMed:19135889; PubMed:19625767). Related references: PMID:18337721 PMID:19135889 PMID:19625767
• It functions as a key histone deacetylase targeting histone H3 at Lys-9, Lys-18, and Lys-56 (H3K9ac, H3K18ac, and H3K56ac), thereby repressing gene expression of transcription factors like NF-kappa-B (PubMed:19625767; PubMed:21362626; PubMed:23892288). Related references: PMID:19625767 PMID:21362626 PMID:23892288
• SIRT6 promotes DNA double-strand break repair by recognizing DSB sites, recruiting repair proteins such as SMARCA5/SNF2H, and deacetylating histones H3K9 and H3K56 (PubMed:23911928; PubMed:31995034; PubMed:32538779). It also deacetylates non-histone repair proteins like DDB2 and p53/TP53 (PubMed:29474172; PubMed:32789493). Related references: PMID:23911928 PMID:31995034 PMID:32538779 PMID:29474172 PMID:32789493
• In telomere maintenance, SIRT6 catalyzes deacetylation of histone H3 in telomeric chromatin, regulating telomere position effect and movement upon DNA damage (PubMed:18337721; PubMed:19625767; PubMed:21847107). Related references: PMID:18337721 PMID:19625767 PMID:21847107
• SIRT6 is a tumor suppressor that represses glycolysis by inhibiting the Warburg effect through deacetylation of HIF1A and PKM2, and it regulates gluconeogenesis and fatty acid oxidation (PubMed:23217706; PubMed:26787900; PubMed:23142079; PubMed:25009184). Related references: PMID:23217706 PMID:26787900 PMID:23142079 PMID:25009184
• The protein localizes predominantly in the nucleus, at chromosomes and telomeres, and also in the endoplasmic reticulum. It is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination, which modulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 256–355 of human SIRT6, a region that may be critical for antibody specificity. Validate the antibody in your specific experimental system using appropriate positive and negative controls.
• For Western blot, the predicted molecular weight is 36–39 kDa; multiple bands representing different isoforms or post-translational modifications may be observed. Optimize sample preparation, gel percentage, and transfer conditions accordingly.
• For ELISA, perform a titration series to determine the optimal antibody dilution. Pair with a suitable detection system appropriate for rabbit monoclonal antibodies.

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