Anti-SIRT5 (SIR2L5) Rabbit Polyclonal Antibody for WB, ELISA - Q9NXA8

Core Product Specifications and Parameters

Biological Background: SIRT5 Function and Localization

• SIRT5 is an NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Related references: PMID:21908771, PMID:22076378, PMID:24703693
• It activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting by mediating desuccinylation and deglutarylation. Related references: PMID:22076378, PMID:24703693
• SIRT5 activates SOD1 by desuccinylation, leading to reduced reactive oxygen species. Related references: PMID:24140062
• It activates SHMT2 by mediating its desuccinylation. Related references: PMID:29180469
• SIRT5 modulates ketogenesis through the desuccinylation and activation of HMGCS2.
• The protein has weak NAD-dependent deacetylase activity, but this may not be physiologically relevant; it can deacetylate cytochrome c in vitro.
• SIRT5 localizes to the mitochondrial matrix, intermembrane space, cytosol, and nucleus, reflecting its dynamic functional roles.
• It is a widely expressed zinc-dependent enzyme, indicative of fundamental metabolic regulation across tissues.

Experimental Guidance and Technical Tips

• For western blot, consider using mitochondrial-enriched fractions or whole cell lysates from human cell lines, as SIRT5 is ubiquitously expressed.
• The predicted molecular weight is ~34 kDa; validate with a positive control and appropriate molecular weight markers.
• In ELISA, the immunogen (aa 216-310) confers specificity; pairing with a detection antibody targeting a distinct epitope may improve sensitivity.
• As a polyclonal antibody, lot-to-lot variability can occur; titrate and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides researchers and diagnostic manufacturers with reliable access to SIRT5 antibodies and related IVD raw materials, supporting metabolic and mitochondrial research. As a one-stop sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and customized solutions to accelerate your assay development from concept to clinic.