Anti-SIRT5 Rabbit Monoclonal Antibody for WB and ELISA - Q9NXA8

Core Product Specifications and Parameters

Biological Background: SIRT5 Function and Localization

• SIRT5 is a mitochondrial NAD-dependent protein deacylase that primarily functions as a lysine demalonylase, desuccinylase, and deglutarylase, specifically removing malonyl, succinyl, and glutaryl groups from target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Related references: PMID:21908771, PMID:22076378, PMID:24703693
• It activates carbamoyl phosphate synthetase 1 (CPS1) by desuccinylation and deglutarylation, thereby contributing to the regulation of blood ammonia levels during prolonged fasting (PubMed:22076378, PubMed:24703693). Related references: PMID:22076378, PMID:24703693
• It activates superoxide dismutase 1 (SOD1) through desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Related references: PMID:24140062
• It activates serine hydroxymethyltransferase 2 (SHMT2) by desuccinylation (PubMed:29180469). Related references: PMID:29180469
• It modulates ketogenesis by desuccinylating and activating 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2) (By similarity).
• SIRT5 exhibits weak NAD-dependent deacetylase activity; however, this may not be physiologically relevant (PubMed:21908771). Related references: PMID:21908771
• SIRT5 is primarily localized in the mitochondrial matrix and intermembrane space, but can also be found in the cytosol and nucleus.
• It is widely expressed across tissues and belongs to the sirtuin family, containing a zinc-binding domain.

Experimental Guidance and Technical Tips

• For Western blot, consider using a mitochondrial fraction or whole cell lysate and validate the expected band at approximately 34kDa.
• For ELISA, the antibody may be used for direct or sandwich ELISA, but optimal conditions require empirical determination.
• Cross-reactivity with human, mouse, and rat is reported; verify in your experimental system.
• The immunogen spans amino acids 37-310, covering the catalytic domain; therefore, the antibody is expected to recognize full-length SIRT5.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop sourcing for IVD raw materials, including validated antibodies like this anti-SIRT5 monoclonal antibody for metabolic and mitochondrial research. We provide bulk reagents, custom antibody development, and technical support to streamline your diagnostic development from concept to clinic.