Anti-SIRT4 Polyclonal Antibody for IF/ICC, ELISA - Q9Y6E7

Core Product Specifications and Parameters

Biological Background: SIRT4 Function and Localization

• SIRT4, also known as SIR2L4, is a mitochondrial NAD-dependent protein lipoamidase, biotinylase, deacetylase, and ADP-ribosyltransferase.
• SIRT4 catalyzes the removal of lipoyl and biotinyl groups more efficiently than acetyl groups, inhibiting pyruvate dehydrogenase complex (PDH) through hydrolysis of lipoamide from the E2 component DLAT.
  Related references:
  PMID:24052263 PMID:25525879
• It mono-ADP-ribosylates mitochondrial glutamate dehydrogenase 1 (GLUD1), inhibiting its activity and negatively regulating glutamine metabolism in response to DNA damage, leading to cell cycle arrest.
  Related references:
  PMID:16959573 PMID:17715127
• SIRT4 acts as a tumor suppressor; mTORC1 signaling represses SIRT4 expression, promoting anaplerosis and cell proliferation.
  Related references:
  PMID:23562301 PMID:23663782
• It mediates deacetylation of MLYCD at Lys-471, inhibiting its activity and thereby regulating lipid homeostasis.
• SIRT4 controls fatty acid oxidation by inhibiting PPARA transcriptional activation, likely through modulating NAD+ levels and disrupting SIRT1-PPARA interaction.
  Related references:
  PMID:24043310
• This protein is widely expressed, with prominent levels in vascular smooth muscle, striated muscle, and insulin-producing pancreatic beta-cells.
• It localizes to the mitochondrial matrix and is a zinc-dependent, NAD-dependent enzyme.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-200) of human SIRT4; consider this epitope specificity when designing detection systems.
• For IF/ICC, optimize mitochondrial staining using appropriate fixation (e.g., 4% PFA) and permeabilization methods; validate with human-derived cell lines.
• In ELISA, this polyclonal antibody can be used as a capture or detection reagent; pair with a specific monoclonal antibody for sandwich assays if needed.
• As a polyclonal antibody, it may recognize multiple epitopes, offering robust signal but requiring careful validation for target specificity in complex samples.
• Confirm reactivity solely with human SIRT4; cross-reactivity with other species has not been reported.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, technical services, and consulting, accelerating transitions from concept to clinic. With a focus on mitochondrial metabolism and oncology targets like SIRT4, CamelBio offers validated antibodies, optimized pair screening, bulk ancillary reagents, and rare-target raw-material supply to diagnostic manufacturers and research laboratories. Streamline your development with one-stop access to high-quality reagents and expert support.