Anti-SIRT3 Rabbit Polyclonal Antibody [KO Validated] for WB and ELISA - Q9NTG7

Core Product Specifications and Parameters

Biological Background: SIRT3 Function and Localization

• SIRT3 is a mitochondrial NAD-dependent protein deacetylase that regulates cellular energy metabolism by deacetylating key lysine residues on target proteins, including ACSS1, IDH, GDH, SOD2, and ATP synthase subunits. Related references: PMID:12186850, PMID:12374852, PMID:16788062
• It additionally functions as a protein-lysine deacylase, mediating debenzoylation (e.g., of PPIF and ACLY) and delactylation (e.g., of CCNE2 and histone H4K16). Related references: PMID:39524354, PMID:36896611, PMID:37720100
• In response to metabolic stress, SIRT3 deacetylates FOXO3 and recruits mitochondrial RNA polymerase to promote mtDNA transcription. Related references: PMID:23283301
• SIRT3 regulates hepatic lipogenesis by activating AMPK signaling, which downregulates SREBP transcriptional activities.
• Localized to the mitochondrial matrix, SIRT3 is widely expressed across tissues.
• The protein contains a transit peptide for mitochondrial import and a zinc-binding domain, with alternative splicing producing additional isoforms.

Experimental Guidance and Technical Tips

• The antibody was raised against a peptide within the C-terminal region (aa 299-399), which is conserved in human, mouse, and rat, supporting the observed cross-reactivity.
• For WB, validate in the relevant tissue or cell lysate; expect a band at approximately 44 kDa.
• For ELISA, consider using the immunogen peptide as a positive control to confirm reactivity.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For SIRT3 research in mitochondrial metabolism and energy regulation, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our supply network also supports rare target raw-material sourcing, ensuring a reliable procurement solution for your IVD development.