Anti-SIRT2 Rabbit Polyclonal Antibody (pAb) for WB, IHC-P, IF/ICC, ELISA - Q8IXJ6

Core Product Specifications and Parameters

Biological Background: SIRT2 Function and Localization

• SIRT2 encodes a NAD-dependent protein deacetylase (sirtuin-2) that also functions as a protein-lysine deacylase, removing acetyl, benzoyl, methacryl, myristoyl, and palmitoyl groups from target proteins, including histones, α-tubulin, transcription factors, and metabolic enzymes. Related references: PMID:12620231, PMID:16648462, PMID:18249187, PMID:30154464, PMID:34961760, PMID:25704306, PMID:29239724, PMID:32103017
• Plays a pivotal role in cell cycle progression and genomic stability by functioning in the antephase checkpoint, preventing premature mitotic entry under microtubule stress, and positively regulating the anaphase promoting complex/cyclosome (APC/C) via deacetylation of CDC20 and FZR1. Related references: PMID:12697818, PMID:16909107, PMID:17488717, PMID:22014574
• Modulates chromatin structure and transcription by specifically deacetylating histone H4 at Lys-16 (H4K16ac) and histone H3 at Lys-56 (H3K56ac) during mitosis, facilitating H4K20 methylation; also associates with transcription start sites and enhancers of active genes. Related references: PMID:23468428, PMID:20587414
• Localizes to multiple subcellular compartments including the nucleus, cytoplasm, centrosome, spindle, midbody, and cell projections; its dynamic shuttling is critical for coordinating cell cycle-dependent functions and stress responses.
• Widely expressed, with isoform 1 prominent in heart, liver, and skeletal muscle, and isoform 2 enriched in the cerebral cortex; down-regulated in many gliomas and several other cancers, consistent with a tumor suppressor role.
• Participates in autophagy regulation by deacetylating FOXO1, and in metabolic control by stabilizing phosphoenolpyruvate carboxykinase PCK1 and activating glucose-6-phosphate dehydrogenase G6PD to manage glucose homeostasis and oxidative stress. Related references: PMID:20543840, PMID:21726808, PMID:24769394
• Deacetylates α-tubulin at Lys-40, thereby regulating microtubule dynamics, neuronal motility, oligodendroglial arborization, and neurite outgrowth; this activity is inhibited by phosphorylation at Ser-368 by the cyclin E-CDK2 complex. Related references: PMID:18332217, PMID:18995842, PMID:17488717
• Involved in innate immunity: upon Listeria monocytogenes infection, SIRT2 deacetylates histone H3 at Lys-18, dampening transcriptional activity to promote late infection stages. Related references: PMID:23908241

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 300–350 near the C-terminus of human SIRT2; consider that isoforms or splice variants may differ in this region. Validate reactivity in your specific sample system.
• For Western blot (WB), expect a major band at approximately 43 kDa; use appropriate positive and negative controls. Optimize lysis and loading conditions to clearly resolve SIRT2.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), the antibody may detect nuclear and cytoplasmic staining depending on cell type and conditions; include antigen retrieval steps and confirm localization with known markers.
• This polyclonal antibody recognizes human, mouse, and rat SIRT2. Cross-reactivity with other species should be experimentally tested if required.
• For ELISA applications, consider pairing with a suitable capture/detection system; preliminary titration is recommended to establish optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering a comprehensive pipeline from discovery to clinical application. This Anti-SIRT2 Rabbit Polyclonal Antibody serves epigenetic and oncology research, and CamelBio also provides validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary supplies, and rare target sourcing to streamline your assay development. Count on us to bridge the gap between concept and clinic with reliable, one-stop procurement solutions.