Anti-SIRT2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q8IXJ6

Core Product Specifications and Parameters

Biological Background: SIRT2 Function and Localization

• SIRT2 is an NAD-dependent protein deacetylase that removes acetyl groups from internal lysine residues of histones, alpha-tubulin, and numerous transcription factors, thereby modulating their functions. Related references: PMID:12620231, PMID:16648462, PMID:18249187
• SIRT2 plays a key role in cell cycle progression and genomic stability. It functions in the antephase checkpoint to prevent premature mitotic entry, and it positively regulates the APC/C complex by deacetylating CDC20 and FZR1, promoting mitosis. Related references: PMID:12697818, PMID:16909107, PMID:17488717, PMID:22014574
• SIRT2 deacetylates histone H4 at Lys-16 (H4K16ac) between the G2/M transition and metaphase, enabling subsequent H4K20 methylation, and also deacetylates histone H3 at Lys-56 (H3K56ac) during the G2/M transition, thereby regulating chromatin compaction and epigenetic signaling. Related references: PMID:23468428, PMID:20587414
• SIRT2 is involved in metabolic regulation: it deacetylates and stabilizes PCK1 to control blood glucose, activates G6PD in the pentose phosphate pathway to produce NADPH and counteract oxidative stress, and inhibits adipogenesis while promoting lipolysis. Related references: PMID:21726808, PMID:24769394, PMID:20543840
• SIRT2 also functions as a protein lysine deacylase, removing benzoyl, methacryl, myristoyl, and palmitoyl groups from target proteins like ARF6 and KRAS, regulating their membrane localization. Related references: PMID:30154464, PMID:34961760, PMID:25704306, PMID:29239724, PMID:32103017
• SIRT2 localizes to the nucleus, cytoplasm, perinuclear region, cytoskeleton (centrosome, spindle, midbody), and cell projections, consistent with its roles in mitosis, cell motility, and myelination. Two major isoforms exist: Isoform 1 is expressed in heart, liver, and skeletal muscle; Isoform 2 is strongly expressed in brain cortex. SIRT2 is frequently down-regulated in gliomas and certain cancers, and is considered a tumor suppressor.

Experimental Guidance and Technical Tips

• For western blotting (WB), a band near 43 kDa is expected for the canonical SIRT2 isoform. Alternative splicing and post‑translational modifications may yield additional bands; validate in your sample system.
• For immunohistochemistry (IHC-P), consider the broad subcellular distribution of SIRT2 and optimize antigen retrieval protocols accordingly. Tissues with high SIRT2 expression (e.g., liver, brain) can serve as positive controls.
• Given SIRT2’s cell cycle-dependent functions, synchronization of cell cultures may enhance detection in proliferation studies.
• For ELISA, ensure the capture/detection antibody format is compatible with the peptide‑immunized monoclonal antibody; note that the immunogen lies in the C‑terminal region of the protein.
• This antibody detects human, mouse, and rat SIRT2; cross‑reactivity with other species or sirtuin family members should be evaluated empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials for diagnostic and research applications, including validated monoclonal antibodies like this anti‑SIRT2 rabbit mAb. We support your development pipeline with optimized antibody pairs for sandwich ELISA, bulk ancillary reagents, and sourcing assistance for rare targets in the cell cycle, epigenetics, and metabolic disease areas. Whether you need custom antibody services or off‑the‑shelf raw materials, CamelBio streamlines your path from concept to clinic.