Anti-SIRT1 Rabbit Monoclonal Antibody (KO Validated) for WB, IF/ICC, ELISA - Q96EB6

Core Product Specifications and Parameters

Biological Background: SIRT1 Function and Localization

• SIRT1 is an NAD-dependent protein deacetylase that serves as a key metabolic sensor, linking transcriptional regulation to intracellular energy status and coordinating diverse cellular processes such as cell cycle, DNA damage response, metabolism, apoptosis, and autophagy.
  Related references:
  PMID:11672523, PMID:12006491, PMID:14976264
• It modulates chromatin function through deacetylation of histones H1, H2A, and H3, and promotes alterations in histone methylation, leading to transcriptional repression.
  Related references:
  PMID:15469825
• SIRT1 deacetylates a broad range of transcription factors and co-regulators, including p53, FOXO1/3/4, NF-κB, PGC-1α, and others, thereby regulating target gene expression both positively and negatively.
  Related references:
  PMID:14976264, PMID:14980222, PMID:15152190, PMID:11672523, PMID:12006491, PMID:15692560, PMID:16892051
• It plays critical roles in DNA damage response and repair by deacetylating repair proteins such as XRCC6/Ku70, WRN, APEX1, and NBN, facilitating their recruitment or activity; it also represses DNA repair gene expression.
  Related references:
  PMID:15205477, PMID:16998810, PMID:17334224, PMID:20097625, PMID:19934257
• SIRT1 is a component of the eNoSC complex and is involved in rDNA silencing, heterochromatin formation, and telomere length regulation, contributing to genomic stability.
  Related references:
  PMID:18485871, PMID:21504832, PMID:15469825, PMID:18004385
• Subcellular localization includes the nucleus, PML bodies, cytoplasm, and mitochondria, with nuclear-cytoplasmic shuttling observed in response to various signals. Implicated in circadian rhythm regulation by deacetylating core clock proteins and histones at clock gene promoters.
  Related references:
  PMID:18662546
• Alternative splicing produces multiple isoforms, including a catalytically inactive isoform (75SirT1) that may regulate apoptosis, and the two major isoforms cooperate to regulate p53 activity.
• In addition to deacetylation, SIRT1 acts as a protein-lysine deacylase mediating delactylation, depropionylation, and decrotonylation of target proteins such as TEAD1, YAP1, and histones.
  Related references:
  PMID:28497810, PMID:38512451

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; consider using peptide competition assays to confirm specificity in your experimental system.
• For WB, the predicted molecular weight is ~82 kDa; optimize blocking and antibody dilution for your cell lysates (suggest starting at 1:1000 dilution per standard protocols).
• For IF/ICC, fixation and permeabilization conditions may affect epitope accessibility; test both paraformaldehyde and methanol fixation.
• For ELISA, the antibody may be used for capture or detection, but pair validation with a suitable matched antibody pair is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a B2B sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies (including KO-validated options like this SIRT1 mAb), bulk ancillary reagents, and rare-target raw-material sourcing to support your metabolic, epigenetic, and oncology research applications. Let CamelBio streamline your supply chain from discovery to diagnostic development.