Anti-SIRT1 Polyclonal Antibody for WB, IP, IHC-P, ELISA - Q96EB6

Core Product Specifications and Parameters

Biological Background: SIRT1 Function and Localization

• SIRT1 is an NAD-dependent protein deacetylase belonging to the sirtuin family, linking transcriptional regulation directly to intracellular energetics and metabolic status (PubMed:11672523, PubMed:12006491, PubMed:14976264).
  Related references: PMID:11672523, PMID:12006491, PMID:14976264
• It modulates chromatin function by deacetylating histones H3, H4, and H2A, promoting transcriptional repression and heterochromatin formation (PubMed:15469825).
  Related references: PMID:15469825
• Deacetylates the tumor suppressor p53 at Lys-382, impairing its transcriptional pro-apoptotic activity and modulating cellular senescence (PubMed:11672523, PubMed:12006491, PubMed:22542455).
  Related references: PMID:11672523, PMID:12006491, PMID:22542455
• Functions in autophagy by deacetylating key autophagy proteins ATG5, ATG7, and MAP1LC3B/LC3 (PubMed:18296641).
  Related references: PMID:18296641
• Plays a role in DNA repair by deacetylating and recruiting repair factors such as XRCC6/Ku70 and NBN to damaged sites (PubMed:15205477, PubMed:16998810, PubMed:17334224).
  Related references: PMID:15205477, PMID:16998810, PMID:17334224
• Localizes to multiple cellular compartments including the nucleus, PML bodies, cytoplasm, and mitochondria, reflecting its diverse functions.
• Widely expressed across tissues, with critical roles in metabolism, development, and stress responses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 448-747 of human SIRT1 (C-terminal region), suggesting the antibody may recognize epitopes in this domain. Verify recognition in your target species if cross-reactivity is uncertain.
• For Western blot, the predicted molecular weight is ~82 kDa; however, post-translational modifications may cause shifts. Include appropriate controls.
• For immunohistochemistry (paraffin), antigen retrieval conditions may need optimization; the antibody has been reported for IHC-P.
• This polyclonal antibody is suitable for immunoprecipitation and ELISA; titrate the antibody for each application in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers focusing on SIRT1 and related metabolic or epigenetic pathways, we offer comprehensive sourcing solutions from concept to clinic. Leverage our expertise to obtain high-quality raw materials and technical support for your assay development needs.