Anti-SIRT1 Polyclonal Antibody for WB, ELISA - Q923E4

Core Product Specifications and Parameters

Biological Background: SIRT1 Function and Localization

• SIRT1 is an NAD-dependent protein deacetylase that links transcriptional regulation to intracellular energetics, coordinating cell cycle, DNA damage response, metabolism, apoptosis, and autophagy. Related references: PMID:11250901, PMID:11672522, PMID:12651913
• It modulates chromatin function through deacetylation of histones (H3, H4, H2A, H1-4) and is a component of the eNoSC complex, which silences rDNA in response to energy status. Related references: PMID:18004385
• SIRT1 deacetylates 'Lys-382' of p53/TP53, impairing its pro-apoptotic transcriptional activity and modulating cell senescence. Related references: PMID:11672522, PMID:12960381
• It regulates metabolism by deacetylating FOXO1 (enhancing gluconeogenesis) and PPARGC1A (activating fatty acid oxidation in muscle under low glucose). Related references: PMID:15220471, PMID:15788402, PMID:15716268
• SIRT1 regulates autophagy, presumably by deacetylating ATG5, ATG7, and MAP1LC3B/ATG8. Related references: PMID:18296641, PMID:21189328
• It plays a critical role in circadian rhythm by deacetylating core clock proteins (BMAL1, PER2) and histones, contributing to circadian chromatin remodeling. Related references: PMID:18662546, PMID:18662547, PMID:19299583
• SIRT1 localizes to the nucleus, PML bodies, cytoplasm, and mitochondria, shuttling between compartments in response to cellular signals.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 638-737) of mouse SIRT1. For Western blot, the antibody detects bands at ~80 kDa and ~60 kDa, potentially representing full-length and a shorter isoform (e.g., 75SirT1). Use appropriate loading controls and validate band specificity with siRNA knockdown or knockout lysates if available.
• SIRT1 is widely expressed but at low levels in liver and skeletal muscle; consider using tissue extracts known to express SIRT1 (e.g., brain, testis) for initial validation.
• For ELISA applications, the peptide immunogen may provide good direct or capture detection sensitivity; optimize coating conditions and antibody dilutions.
• Cross-reactivity has been confirmed for mouse; if using in other species, verify sequence homology of the immunogen region and perform species-specific validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for global diagnostic manufacturers and research laboratories. Our SIRT1 antibody supports development of assays in areas such as metabolic syndrome, cancer epigenetics, and aging research. We also specialize in rare-target antibody sourcing and customized raw-material supply to accelerate your diagnostic pipeline from concept to clinic.