Anti-SIPA1 Rabbit Polyclonal Antibody for WB and ELISA - Q96FS4

Core Product Specifications and Parameters

Biological Background: SIPA1 Function and Localization

• SIPA1 (Signal-induced proliferation-associated protein 1), also known as GTPase-activating protein Spa-1 or p130 SPA-1, is a 112 kDa protein encoded by the SIPA1 gene (Gene ID: 6494).
• Functions as a GTPase activator for Rap1 and Rap2, converting them to the inactive GDP-bound state.
  Related references: PMID:9346962
• Involved in regulating cell cycle progression (by similarity).
• Localizes to the nucleus, cytoplasm, perinuclear region, and endomembrane system.
• Highly expressed in lymphoid tissues (thymus, spleen, peripheral blood lymphocytes) and also significantly in the spinal cord.
• Contains a coiled-coil domain, is subject to phosphorylation, and belongs to the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 763–1042 of human SIPA1, targeting the C-terminal region; therefore, the antibody may detect full-length SIPA1 as well as any C-terminal fragments or isoforms containing this sequence.
• For western blot (WB), expect a major band at approximately 112 kDa. Optimize sample preparation and electrophoresis conditions to resolve this high-molecular-weight protein.
• Confirm species cross-reactivity in the relevant cellular context; the antibody is reported to react with human and mouse SIPA1.
• For ELISA, consider using the antibody as a capture or detection reagent following standard titration to determine optimal working concentrations.
• Lysates from lymphoid tissues (e.g., thymus, spleen) or cell lines with known SIPA1 expression may serve as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with seamless access to high-quality raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-SIPA1 reagent, and bulk ancillary supplies. With a focus on signal transduction and cell proliferation targets, we streamline procurement of rare-target raw materials and offer consulting support from concept to clinic. Let CamelBio be your trusted partner in advancing diagnostic development.