Anti-Sin3A Rabbit Polyclonal Antibody for WB - Q96ST3

Core Product Specifications and Parameters

Biological Background: Sin3A Function and Localization

• Encoded by the SIN3A gene, Sin3a (Paired amphipathic helix protein Sin3a, histone deacetylase complex subunit Sin3a) is a transcriptional corepressor that mediates repression through interactions with various transcription factors.
• Acts as a corepressor for REST, interacts with MXI1 to repress MYC-responsive genes and antagonize MYC oncogenic activities, and interacts with MXD1-MAX heterodimers to tether SIN3A to DNA.
• Cooperates with OGT to repress transcription in parallel with histone deacetylation; involved in circadian rhythm control by repressing circadian target genes like PER1 through histone deacetylation as part of the large PER complex.
• Cooperates with FOXK1 to regulate cell cycle progression likely by repressing cell cycle inhibitor gene expression (By similarity).
• Required for cortical neuron differentiation and callosal axon elongation (By similarity).
• Located in the nucleus and nucleolus.
• Highly expressed in the developing brain, particularly in the ventricular zone of various cortical regions.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation (based on UniProt keywords).

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to the N-terminal region (aa 1-120) of human Sin3A. For applications requiring native conformation (e.g., IP, IF), confirm that the epitope is accessible under native conditions.
• For Western blotting, the predicted molecular weight is 145 kDa. Use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• If using for immunoprecipitation, consider cross-linking the antibody to beads to avoid interference from the heavy chain which co-migrates near 145 kDa.
• For immunofluorescence, nuclear localization supports staining patterns; permeabilization may be necessary for nucleolar access.
• The antibody detects human, mouse, and rat Sin3A, suggesting conserved epitopes; for other species, check sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with reliable antibodies, antigen pairs, and ancillary reagents. This Sin3A polyclonal antibody, applicable to transcriptional regulation and circadian rhythm studies, exemplifies our commitment to supplying high-quality tools for diverse research areas. We also offer custom sourcing for rare targets and bulk supply options to accelerate your development from concept to clinic.