Anti-Sin3A Monoclonal Antibody for WB, ELISA - Q96ST3

Core Product Specifications and Parameters

Biological Background: Sin3A Function and Localization

• Sin3A (Paired amphipathic helix protein Sin3a) is a core component of the histone deacetylase (HDAC) complex, functioning as a transcriptional corepressor.
• It directly interacts with various transcription factors, such as REST, MXI1, and MXD1-MAX heterodimers, to repress MYC-responsive genes and antagonize MYC oncogenic activities.
• Sin3A is involved in the regulation of circadian rhythms by mediating the transcriptional repression of circadian target genes (e.g., PER1) through histone deacetylation, in cooperation with the large PER complex.
• As suggested by its name, Sin3A contains paired amphipathic helix domains that facilitate interactions with multiple transcription factors and corepressors.
• It localizes primarily to the nucleus and nucleolus, consistent with its role in transcriptional regulation.
• Tissue-specific expression: Sin3A is highly expressed in the developing brain, particularly in the ventricular zone of various cortical regions, where it is required for cortical neuron differentiation and callosal axon elongation.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation (isopeptide bond), indicating multiple layers of regulation.
• Mutation or dysfunction of SIN3A is associated with disease variants, including intellectual disability and dwarfism (WITKOS syndrome).

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human Sin3A. This region may be involved in protein-protein interactions; consider potential epitope masking in native complexes.
• For Western blot (WB), the predicted molecular weight of Sin3A is approximately 145 kDa. Ensure proper gel electrophoresis conditions for high molecular weight proteins and validate the antibody with appropriate positive and negative controls in the relevant sample system.
• In ELISA applications, consider titrating the antibody against the immunogen peptide or recombinant Sin3A protein to determine optimal working concentrations. Perform cross-reactivity tests with lysates from human and mouse tissues as indicated.
• Given Sin3A's role in transcriptional regulation and chromatin remodeling, this antibody may be useful for studying protein interactions (e.g., Co-IP) but such applications have not been validated; independent validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. This Sin3A rabbit monoclonal antibody targets a key transcriptional regulator, supporting research in epigenetics, developmental biology, and oncology. Leverage CamelBio's comprehensive supply chain to accelerate your diagnostic development from concept to clinic.