Anti-SIM2 Rabbit Polyclonal Antibody for WB, ELISA - Q14190

Core Product Specifications and Parameters

Biological Background: SIM2 Function and Localization

• Single-minded homolog 2 (SIM2) is a transcription factor belonging to the class E basic helix-loop-helix (bHLH) family, also known as bHLHe15, and is encoded by the SIM2 gene.
• SIM2 is proposed to function as a master regulatory gene in central nervous system (CNS) development, often acting in cooperation with the aryl hydrocarbon receptor nuclear translocator (ARNT) (UniProt).
• Consistent with its developmental role, SIM2 contains bHLH and PAS domains that facilitate DNA binding and protein dimerization, enabling it to regulate target gene expression.
• SIM2 is localized primarily to the nucleus, supporting its role as a transcription factor involved in neurogenesis and differentiation.
• The protein undergoes alternative splicing, producing multiple isoforms that may have tissue-specific or context-dependent functions.
• UniProt keywords highlight SIM2’s involvement in developmental processes, transcriptional regulation, and its identification in proteomics studies, underscoring its broad regulatory potential.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 342-525 of human SIM2, a region likely encompassing the C-terminal portion of the protein. This design may favor recognition of native SIM2 across multiple species given the sequence conservation among human, mouse, and rat.
• For western blot (WB), the predicted molecular weight of SIM2 is approximately 73 kDa. Use appropriate protein loading controls and validate antibody specificity using SIM2-positive cell lines or tissues from CNS regions.
• In ELISA applications, consider optimizing the coating concentration of the recombinant antigen and including appropriate negative controls to minimize background.
• Since this is a polyclonal antibody, performance may vary between lots; it is advisable to confirm reactivity in your specific experimental system and consider pre-absorption steps if cross-reactivity is observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, bridging the gap from concept to clinical application. This anti-SIM2 polyclonal antibody supports neuroscience and developmental biology research, and we can assist with sourcing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need rare-target raw materials or consistent supply for diagnostic assay development, CamelBio offers tailored procurement solutions to accelerate your project.