Anti-SIK3 Polyclonal Antibody for WB and ELISA - Q9Y2K2

Core Product Specifications and Parameters

Biological Background: SIK3 Function and Localization

• SIK3 (Serine/threonine-protein kinase SIK3), also known as Salt-inducible kinase 3 (SIK-3) or QSK, is a member of the AMPK-related kinase family and plays key roles in metabolic signaling and cytoskeletal regulation.
• Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis. Related references: PMID:30232230
• Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3, thereby influencing glucose metabolism and transcription.
• Prevents HDAC4 translocation to the nucleus, thereby modulating gene expression programs important for cell survival and differentiation.
• Subcellularly localized to the cytoplasm, consistent with its role as a cytoplasmic kinase.
• Expressed in chondrocytes and implicated in skeletal development; mutations in SIK3 have been associated with skeletal dysplasias.
• Contains characteristic kinase domain signatures, including ATP-binding and magnesium coordination sites, and is subject to phosphorylation and methylation.
• Multiple isoforms exist due to alternative splicing, which may contribute to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 60–310 of human SIK3, a region that includes part of the kinase domain; this polyclonal antibody may recognize multiple splice variants—validate with appropriate controls.
• For Western blot, a band around 145 kDa is expected in whole‑cell lysates; consider testing samples from chondrocyte-like cell lines or those with active mTOR signaling.
• Human, mouse, and rat cross‑reactivity is reported, making this antibody suitable for cross‑species comparison studies—optimize conditions for each species.
• ELISA applications may require direct coating of the recombinant immunogen or antigen capture; titer and condition optimization is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, including validated polyclonal antibodies like anti-SIK3, optimized antibody pairs, and bulk ancillary reagents for diagnostic development. Whether your work focuses on kinase biomarkers, mTOR pathway targets, or skeletal dysplasia research, we support your project from concept to clinic with reliable sourcing and technical consulting.