Anti-SIK2 Rabbit Polyclonal Antibody for WB, ELISA - Q9H0K1

Core Product Specifications and Parameters

Biological Background: SIK2 Function and Localization

• SIK2 (Qin-induced kinase, Salt-inducible kinase 2) is a serine/threonine-protein kinase belonging to the AMPK subfamily, playing critical roles in metabolic regulation, autophagy, and immune responses.
• Core functions include phosphorylation of IRS1 at Ser-794, modulating insulin signal transduction, and inhibition of CREB-dependent transcription by phosphorylating TORC coactivators. Related references: PMID:15454081
• SIK2 phosphorylates EP300, inhibiting its histone acetyltransferase activity, thereby regulating the DNA-binding ability of transcription factors such as PPARA and MLXIPL involved in fatty acid oxidation and glucose metabolism. Related references: PMID:21084751 PMID:26983400
• The kinase is also implicated in thymic T-cell development, as indicated by similarity data.
• Subcellularly, SIK2 is localized in the cytoplasm and associated with the endoplasmic reticulum membrane.
• Post-translational modifications include acetylation and phosphorylation; the protein contains conserved ATP-binding and metal-binding domains.
• With a molecular weight of approximately 104 kDa, SIK2 is widely expressed and is being investigated as a potential target in metabolic disorders and cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 827-926) of human SIK2, suggesting the antibody may recognize full-length protein and possibly C-terminal variants.
• For Western blot, a band at approximately 104 kDa is expected; consider optimizing protein loading and antibody dilution for your specific sample type.
• Validate the antibody in lysates from human, mouse, or rat tissues known to express SIK2, such as liver, adipose, or immune cells.
• For ELISA, consider using the recombinant protein immunogen as a positive control and testing a range of coating and detection conditions.
• Due to SIK2’s roles in insulin signaling and autophagy, experiments in metabolic or cancer models may require careful normalization to relevant loading controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, offering comprehensive solutions from early-stage R&D to clinical validation. Our portfolio includes rigorously validated antibody pairs, optimized polyclonal and monoclonal antibodies like this SIK2 rabbit pAb, and a wide range of ancillary reagents to support your metabolic and oncology diagnostic development. With expertise in rare target sourcing and bulk supply, CamelBio streamlines your procurement, enabling you to focus on innovation.