Anti-SIK1 Rabbit Polyclonal Antibody for WB, ELISA - P57059

Core Product Specifications and Parameters

Biological Background: SIK1 Function and Localization

• SIK1 (also known as MSK, SNF1LK, or salt-inducible kinase 1) is a serine/threonine-protein kinase that phosphorylates downstream targets including HDAC4, HDAC5, PPME1, SREBF1, and CRTC1/TORC1.
• It regulates hepatic gluconeogenesis and lipogenesis by phosphorylating and inhibiting the CREB coactivators CRTC1/TORC1 and CRTC2/TORC2, as well as SREBF1, thereby suppressing CREB-mediated transcription in response to cAMP signaling. Related references: PMID:29211348
• SIK1 is involved in muscle cell differentiation and cardiomyogenesis by phosphorylating class II histone deacetylases HDAC4 and HDAC5, leading to activation of MEF2 target genes and regulation of cell cycle exit.
• It participates in the regulation of circadian rhythms by attenuating PER1 induction in response to light, via CRTC1/TORC1 phosphorylation.
• Subcellular localization includes both cytoplasm and nucleus, consistent with its role in shuttling and phosphorylating nuclear and cytoplasmic targets.
• SIK1 contains an ATP-binding domain and requires magnesium as a cofactor for its kinase activity; it is classified as a transferase and a member of the serine/threonine-protein kinase family.
• The protein is associated with keywords such as Biological rhythms, Cell cycle, Tumor suppressor, and Disease variant (DEE30, epilepsy).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human SIK1 (NP_775490.2), targeting the N-terminal region; this may allow detection of full-length SIK1 and its isoforms.
• For Western blot, a band at approximately 85 kDa is expected; validate specificity using appropriate positive and negative controls, such as SIK1-overexpressing or knockout cell lysates.
• Cross-reactivity with mouse SIK1 has been confirmed; consider sequence alignment to assess reactivity with other species if needed.
• For ELISA applications, optimal coating and detection antibody concentrations should be determined empirically using the relevant analyte and sample matrix.
• Given SIK1's roles in multiple signaling pathways, consider using specific phosphatase inhibitors during sample preparation to preserve phosphorylation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers comprehensive raw material solutions for diagnostic developers and research laboratories, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-SIK1 antibody supports metabolism, cancer, and circadian rhythm research. With expert support for rare target sourcing, CamelBio streamlines your assay development from concept to clinic.