Anti-SIGMAR1 Rabbit Polyclonal Antibody for WB and ELISA - Q99720

Core Product Specifications and Parameters

Biological Background: SIGMAR1 Function and Localization

• SIGMAR1, also known as sigma non-opioid intracellular receptor 1, SRBP, or SIG1R, is a 25 kDa transmembrane protein encoded by the SIGMAR1 gene (alternative name OPRS1).
• It mediates lipid transport from the endoplasmic reticulum and regulates the biogenesis of lipid microdomains at the plasma membrane, impacting a wide array of cellular functions. Related references: PMID:16472803, PMID:9341151
• SIGMAR1 modulates multiple signaling pathways, including BDNF and EGF signaling, and regulates ion channels such as potassium channels, potentially affecting neurotransmitter release. Related references: PMID:16472803, PMID:9341151
• Together with ANK2, it controls ITP3R-dependent calcium efflux at the endoplasmic reticulum, playing a role in intracellular calcium signaling.
• The protein is necessary for proper mitochondrial axonal transport in motor neurons, particularly retrograde movement of mitochondria, and protects against oxidative stress-induced cell death via interaction with RNF112 (By similarity).
• Subcellular localization includes the endoplasmic reticulum membrane, nucleus envelope, cell membrane, cytoplasmic vesicles, lipid droplets, cell junctions, and growth cones.
• It is widely expressed, with higher levels in liver, colon, prostate, placenta, small intestine, heart, and pancreas, as well as in retinal pigment epithelial cells and alpha-motor neurons (PubMed:23314020). Related references: PMID:23314020
• Clinically, mutations in SIGMAR1 are associated with amyotrophic lateral sclerosis (ALS16) and distal spinal muscular atrophy type 2 (DSMA2), and the protein is implicated in learning, memory, and mood disorders.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 31-80, a region that may be conserved across species; consider verifying sequence homology for your model organism.
• For Western blot, a band at approximately 25 kDa is expected in human and rat samples; optimize blocking and antibody dilution as pre-immune serum may vary between lots.
• For ELISA, the polyclonal nature allows capture-detection pairing, but cross-adsorption against relevant species may be necessary to reduce background.
• The antibody is not validated for immunohistochemistry or immunoprecipitation; if required, test under native and denaturing conditions after confirming target accessibility in your tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to IVD raw materials, technical services, and consultancy. For SIGMAR1-focused neuroscience and cell signaling studies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Reach out to us for rare target raw-material sourcing to support your assay development from concept to clinic.