Anti-SIGLEC9 Polyclonal Antibody for WB, ELISA - Q9Y336

Core Product Specifications and Parameters

Biological Background: SIGLEC9 Function and Localization

• SIGLEC9 (sialic acid-binding Ig-like lectin 9) is a member of the siglec family of immune receptors that recognize sialic acids.
• It functions as a putative adhesion molecule mediating sialic-acid-dependent cell binding, with preference for alpha-2,3- or alpha-2,6-linked sialic acids. The sialic acid recognition site may be masked by cis interactions on the same cell.
• The protein is a transmembrane glycoprotein with an extracellular immunoglobulin-like domain and intracellular signaling motifs.
• Subcellular localization is primarily at the plasma membrane.
• SIGLEC9 is expressed on peripheral blood leukocytes, particularly neutrophils and monocytes, and is found in tissues including liver, bone marrow, placenta, and spleen.
• Post-translational modifications include glycosylation and disulfide bond formation, as indicated by UniProt keywords.
• Alternative splicing generates multiple transcript variants (isoforms).
• The antibody targets the extracellular region (amino acids 210-340), suitable for detecting both surface and total protein forms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment covering amino acids 210-340 of human SIGLEC9. When designing experiments, consider epitope accessibility in native versus denatured conditions.
• For Western blot, a band around 50 kDa is expected. Validate with positive controls (e.g., human leukocyte lysates) and negative controls.
• ELISA can be used for antibody titer determination or antigen detection; optimize coating and detection conditions for your assay.
• Cross-reactivity with mouse and rat is reported; confirm reactivity experimentally if using these species, and check sequence alignment for epitope conservation.
• Avoid freeze-thaw cycles to maintain antibody activity; store as recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD raw material sourcing, offering a comprehensive range of validated antibodies, antigen pairs, and ancillary reagents. For research on immune cell adhesion and sialic acid biology, our anti-SIGLEC9 polyclonal antibody supports assay development and biomarker studies. We streamline procurement from concept to clinic, ensuring timely access to high-quality materials and technical support.