Anti-SIGLEC12 Rabbit Polyclonal Antibody for WB, ELISA - Q96PQ1

Core Product Specifications and Parameters

Biological Background: SIGLEC12 Function and Localization

• SIGLEC12 encodes a putative adhesion molecule that mediates sialic-acid dependent binding to cells, with the sialic acid recognition site possibly masked by cis interactions on the same cell surface.
• It belongs to the sialic acid-binding immunoglobulin-like lectin (Siglec) family, containing immunoglobulin domains and functioning as a lectin.
• Alternative splicing generates at least two isoforms (Long and Short), which show distinct tissue expression patterns.
• The protein is classified as a membrane protein with a signal peptide, transmembrane helix, and disulfide bonds, and it undergoes phosphorylation.
• Isoform Short is highly expressed in spleen, small intestine, and adrenal gland; Isoform Long is highly expressed in spleen, small intestine, and bone marrow.
• Identified as a glycoprotein and a component of proteomics identification studies, indicating its presence in reference proteomes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 20-210), which includes the predicted signal peptide cleavage site and the N-terminal immunoglobulin-like domain.
• For Western blot, the predicted molecular weight of SIGLEC12 is approximately 65 kDa; verify band size with positive control lysates.
• When using this antibody in ELISA, consider pairing with an appropriate detection antibody and validate with recombinant protein or cell lysates.
• Cross-reactivity with mouse suggests potential use in murine samples; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-SIGLEC12 antibody, and bulk ancillary reagents. For research on Siglec-mediated cell adhesion and immune recognition, we provide one-stop sourcing and technical support to accelerate your diagnostic development.