Anti-SIGLEC10 Rabbit Polyclonal Antibody for WB, ELISA - Q96LC7

Core Product Specifications and Parameters

Biological Background: SIGLEC10 Function and Localization

• Sialic acid-binding Ig-like lectin 10 (Siglec-10) is a member of the immunoglobulin superfamily that mediates sialic acid-dependent cell adhesion, preferentially recognizing α2,3- or α2,6-linked sialic acids on glycoconjugates.
• Functions as an inhibitory receptor in immune cells, undergoing ligand-induced tyrosine phosphorylation to recruit phosphatases such as PTPN6/SHP-1 via SH2 domains, thereby dampening B-cell antigen receptor signaling and other activation pathways. Related references: PMID:11284738 PMID:12163025
• In association with CD24, modulates natural killer (NK) cell immune responses and may suppress reactivity to danger-associated molecular patterns (DAMPs) like HMGB1, HSP70, and HSP90. Related references: PMID:25450598
• Multiple isoforms exist due to alternative splicing; isoform 5 is the most abundant in peripheral blood leukocytes, while isoform 4 is specifically expressed in NK cells. Found in lymph node, lung, ovary, appendix, bone marrow, spleen, and brain.
• Primarily located at the cell membrane, but also detected in secreted form and across various membrane compartments.
• Post-translational modifications include glycosylation and phosphorylation; contains disulfide bonds and immunoglobulin-like domains (UniProt keywords: Glycoprotein, Phosphoprotein, Disulfide bond, Immunoglobulin domain).

Experimental Guidance and Technical Tips

• Since the immunogen covers the C‑terminal region (aa 627‑697), the polyclonal antibody may recognize multiple splice isoforms; validate specificity in your target sample system.
• For Western blot, anticipate a band around 77 kDa; glycosylation may shift the apparent molecular weight. Optimize sample preparation, loading amounts, and antibody dilution.
• For ELISA, consider pairing with a matched detection antibody when developing a sandwich assay; polyclonal antibodies offer broad epitope coverage but may require careful optimization of coating and detection conditions.
• Include appropriate controls (e.g., negative cell lines, blocking peptides) to confirm reactivity in human and rat samples, and verify cross‑reactivity if used across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. The SIGLEC10 Rabbit pAb targets a key immune regulatory lectin, supporting research in autoimmunity, inflammation, and oncology. Leverage our sourcing capabilities for rare targets and customize de novo development to accelerate your diagnostic assay pipeline.