Anti-Siglec-8 Monoclonal Antibody for FC - Q9NYZ4

Core Product Specifications and Parameters

Biological Background: Siglec-8 Function and Localization

• Siglec-8 (Sialic acid-binding Ig-like lectin 8) is a member of the sialoadhesin family, also known as SAF-2. It is a single-pass type I membrane protein encoded by the SIGLEC8 gene.
• Functions as a putative adhesion molecule that mediates sialic-acid dependent binding to blood cells. Preferentially binds to alpha-2,3-linked sialic acid and, to a lesser extent, alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Related references: PMID:10625619 PMID:10856141
• Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose, with a preference for Gal-6-sulfated sialyl-Lewis X glycan epitopes. Related references: PMID:27357658
• Expressed specifically on blood cells, namely basophils, mast cells, and eosinophils, making it a key marker for these cell types.
• Subcellularly localized to the plasma membrane.
• Contains immunoglobulin-like domains and is subject to alternative splicing and glycosylation, as indicated by its multiple isoforms and post-translational modifications.

Experimental Guidance and Technical Tips

• The direct conjugate eliminates the need for a secondary antibody, simplifying flow cytometry workflows and reducing background.
• Consider titrating the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Validate expression in relevant samples such as isolated human eosinophils, basophils, or mast cells, as Siglec-8 expression is restricted to these lineages.
• Include appropriate isotype controls to distinguish specific from non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For Siglec-8 research and allergy-related diagnostics, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and production needs.