Anti-SIAH1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q8IUQ4

Core Product Specifications and Parameters

Biological Background: SIAH1 Function and Localization

• SIAH1 (Seven in absentia homolog 1) is a RING-type E3 ubiquitin ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. Related references: PMID:14506261, PMID:14645235, PMID:14654780
• It triggers the degradation of diverse substrates, including transcription regulators (ELL2, MYB, POU2AF1, PML, RBBP8), cell surface receptors (DCC, FLT3), and cytoplasmic signal molecules (KLF10/TIEG1, NUMB). Related references: PMID:10747903, PMID:11146551, PMID:11389839
• SIAH1 confers constitutive instability to HIPK2 through proteasomal degradation, influencing cellular processes. Related references: PMID:18536714, PMID:33591310
• It plays a role in the Wnt signaling pathway by mediating the ubiquitin-dependent degradation of AXIN1. Related references: PMID:28546513, PMID:32430360
• SIAH1 is involved in many cellular processes including apoptosis, tumor suppression, cell cycle regulation, axon guidance, transcription regulation, spermatogenesis, and TNF signaling. Related references: PMID:14506261, PMID:14645235, PMID:14654780
• It localizes to both the cytoplasm and nucleus.
• SIAH1 is widely expressed at a low level in tissues and is down-regulated in advanced hepatocellular carcinomas.
• SIAH1 has partially overlapping functions with its homolog SIAH2. Related references: PMID:14506261, PMID:14645235, PMID:14654780

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human SIAH1 (amino acids 1–313), suggesting the antibody may detect multiple isoforms; consider verifying isoform specificity in your system.
• Predicted protein weight is ~31 kDa; a band near this size can be expected in WB, but consider that post-translational modifications or degradation may produce additional bands.
• For IF/ICC, anticipate both nuclear and cytoplasmic staining patterns; fixation and permeabilization conditions should be optimized accordingly.
• For IHC-P, antigen retrieval may be necessary; validate with positive and negative tissue controls.
• In ELISA, the antibody can be used for direct or capture detection; pairing with a matched detection antibody may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing bridge for IVD raw materials, enabling diagnostic developers to access validated antibodies, bulk ancillary reagents, and customized sourcing solutions for challenging targets. This anti-SIAH1 polyclonal antibody supports research into ubiquitin-proteasome pathways and cancer biology, and CamelBio can assist with matching antibody pairs, large-volume orders, and overall workflow optimization from concept to clinic.