Anti-SIAE Rabbit Polyclonal Antibody for WB and ELISA - Q9HAT2

Core Product Specifications and Parameters

Biological Background: SIAE Function and Localization

• SIAE encodes sialate O-acetylesterase, also known as H-Lse or sialic acid-specific 9-O-acetylesterase, a key enzyme in sialic acid metabolism.
• It catalyzes the removal of 9-O-acetyl ester groups from free diacetylated sialate in the cytosol and from sialylglycoconjugates in lysosomes.
• Together with sialate-O-acetyltransferase, SIAE regulates the balance of acetylated sialoglycoconjugates, which are critical for cell-cell interactions, host-pathogen recognition, and tumor antigenicity (Probable). Related references: PMID:21803834
• Subcellularly, SIAE localizes to both lysosomes and the cytoplasm.
• It is widely expressed in human tissues, with particularly high levels observed in testis, prostate, and colon.
• The Uniprot annotation includes keywords 'Disease variant' and 'Alternative splicing', suggesting that mutations in SIAE may contribute to disease susceptibility and that multiple isoforms exist.
• SIAE is a glycoprotein with serine esterase activity, contains a signal peptide, and has a predicted molecular weight of approximately 58 kDa.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant protein corresponding to amino acids 270-450 of human SIAE, which lies in the C-terminal region. This design may recognize multiple isoforms; therefore, validate specificity in your experimental system.
• For Western blot (WB), a band at approximately 58 kDa is expected. Confirm the molecular weight using appropriate size markers and consider the use of a loading control to ensure equal protein loading.
• When performing ELISA, optimization of coating antigen concentration and blocking conditions is recommended to achieve optimal signal-to-noise ratios.
• Cross-reactivity is reported for human; if working with other species, verify sequence homology and test reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-SIAE antibody, and bulk ancillary reagents. Whether your work focuses on sialic acid metabolism, cell-cell interaction studies, or tumor antigenicity research, CamelBio streamlines your procurement from concept to clinic. With expertise in rare target sourcing and reliable supply chains, we are your trusted partner for scaling up reagent needs.