Anti-SHP/NR0B2 Rabbit Polyclonal Antibody for WB and ELISA - Q15466

Core Product Specifications and Parameters

Biological Background: SHP/NR0B2 Function and Localization

• Encodes nuclear receptor subfamily 0 group B member 2, also known as small heterodimer partner (SHP), an orphan nuclear receptor that lacks a DNA-binding domain.
• Acts as a transcriptional repressor that negatively regulates receptor-dependent signaling pathways by inhibiting transactivation of interacting nuclear receptors. Related references: PMID:22504882
• Inhibits NEUROD1 transcriptional activity on E-box-containing promoters by interfering with the coactivation function of the p300/CBP-mediated transcription complex. Related references: PMID:14752053
• Functions as an essential component of the liver circadian clock, regulating NPAS2-mediated hepatic lipid metabolism through interactions with NR1D1 and RORG.
• Modulates circadian expression of cytochrome P450 (CYP) enzymes, influencing drug metabolism and lipid homeostasis.
• Localizes to both the nucleus and cytoplasm, consistent with its role as a transcriptional regulator and potential cytoplasmic interactions.
• Primarily expressed in the liver, with low levels detected in heart and pancreas; contains methylation sites and is associated with obesity when mutated.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-257, covering nearly the full-length SHP/NR0B2 protein; the polyclonal antibody may detect full-length and potentially truncated forms.
• For western blotting, a band around 28 kDa is expected; consider using liver tissue lysates or SHP-overexpressing cell lines as positive controls.
• Cross-reactivity with mouse and rat is indicated; validate specificity in these species using appropriate controls and sample matrices.
• For ELISA, the recombinant immunogen can serve as a standard; assess linearity and sensitivity with your specific sample type.
• Given the nuclear localization, nuclear extracts or subcellular fractionation may enhance detection in cell-based assays.

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CamelBio is your dedicated partner for IVD raw-material procurement, offering validated antibody pairs, bulk reagents, and rare target sourcing. From concept to clinic, we provide comprehensive support for diagnostic developers and research laboratories focusing on nuclear receptor signaling and metabolic studies.