Anti-SHP2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q06124

Core Product Specifications and Parameters

Biological Background: SHP2 Function and Localization

• SHP2 (Tyrosine-protein phosphatase non-receptor type 11, also known as PTP-1D, PTP-2C, SH-PTP2, SH-PTP3) is a ubiquitously expressed protein tyrosine phosphatase containing two SH2 domains and a catalytic phosphatase domain.
• SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in signal transduction from the cell surface to the nucleus. Related references: PMID:10655584, PMID:14739280, PMID:18559669
• Positively regulates the MAPK signal transduction pathway and dephosphorylates key signaling intermediates such as GAB1, ARHGAP35, and EGFR. Related references: PMID:28074573
• Dephosphorylates ROCK2 at Tyr-722, stimulating its RhoA binding activity, and dephosphorylates CDC73. Related references: PMID:18559669, PMID:26742426
• Modulates PDCD1-mediated T-cell inhibition by dephosphorylating TCR signaling molecules upon recruitment by phosphorylated PDCD1. Related references: PMID:32184441
• Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L. Related references: PMID:19275884
• Localizes to both the cytoplasm and nucleus and is widely expressed, with highest levels in heart, brain, and skeletal muscle.
• Subject to post-translational modifications including phosphorylation and acetylation; mutations are associated with Noonan syndrome and other developmental disorders.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 514–593 of human SHP2 (C-terminal region), which may recognize multiple SHP2 isoforms. Validate the antibody’s reactivity with the target in your experimental system.
• For Western blotting, titrate the antibody to obtain optimal signal-to-noise ratio using a lysate known to express SHP2 (e.g., HeLa or Jurkat cell lysates).
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize fixation and antigen retrieval protocols, and include appropriate controls.
• Confirm cross-reactivity with mouse and rat samples, as expression levels may vary across species and tissues.
• For ELISA, consider using this antibody as a capture or detection reagent, but perform pairing validation and matrix effect testing for your specific assay design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated anti-SHP2 monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents to support your assay development. Whether you need bulk quantities of this SHP2 Rabbit pAb or custom sourcing for other targets in the RAS-MAPK pathway, our global supply chain and technical team ensure reliable, research-to-clinic support.