Anti-SHP2 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q06124

Core Product Specifications and Parameters

Biological Background: SHP2 Function and Localization

• SHP2, encoded by the PTPN11 gene, is a non-receptor tyrosine-protein phosphatase also known as PTP-1D, SH-PTP2, and associated with Noonan syndrome (NS1) and juvenile myelomonocytic leukemia (JMML).
• Acts downstream of various receptor and cytoplasmic protein tyrosine kinases, playing a central role in signal transduction from the cell surface to the nucleus, and positively regulates the MAPK signaling cascade. Related references: PMID:10655584 PMID:14739280 PMID:18559669 PMID:28074573
• Dephosphorylates a broad spectrum of substrates including GAB1, ARHGAP35, EGFR, ROCK2, CDC73, SOX9, and NEDD9/CAS-L, thereby regulating diverse processes such as RhoA activation, ossification, and cell adhesion. Related references: PMID:28074573 PMID:18559669 PMID:26742426 PMID:19275884
• Functions as an effector of PDCD1 (PD-1) in T-cells by dephosphorylating key T-cell receptor (TCR) proximal signaling molecules upon recruitment to phosphorylated PDCD1, leading to inhibition of T-cell responses. Related references: PMID:32184441
• Localizes to both the cytoplasm and nucleus, consistent with its role in transducing extracellular signals to transcriptional machinery.
• Widely expressed across tissues with highest levels observed in heart, brain, and skeletal muscle, indicating its importance in multiple physiological contexts.
• Contains two N-terminal SH2 domains and a C-terminal tyrosine-phosphatase domain, and undergoes post-translational modifications including acetylation and phosphorylation that regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 502–593 within the C‑terminal region of human SHP2, which may support recognition of major isoforms and splice variants.
• For Western blot (WB), a band at approximately 68 kDa is expected in human cell lines such as HeLa or HEK293; however, endogenous expression levels and post‑translational modifications can influence detection, so titrate the antibody empirically.
• In immunoprecipitation (IP) and ELISA, optimize buffer conditions and blocking steps to maintain antigen‑antibody binding.
• Given the human reactivity, validate cross‑reactivity in your specific sample system if other species are to be tested.
• Consider appropriate controls (e.g., phosphatase inhibitors) when studying phosphorylation‑dependent interactions or activity-dependent localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes integrated access to IVD raw materials, technical services, and consulting. For SHP2‑related assay development and signal transduction research, our portfolio includes validated antibody pairs, curated monoclonal antibodies, and bulk ancillary reagents. Whatever your stage—from early feasibility to clinical validation—CamelBio streamlines sourcing so you can focus on innovation.