Anti-SHP2 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q06124

Core Product Specifications and Parameters

Biological Background: SHP2 Function and Localization

• SHP2 (also known as PTPN11, PTP-1D, PTP-2C, SH-PTP2, SH-PTP3) is a 68 kDa non-receptor tyrosine phosphatase encoded by the PTPN11 gene in humans.
• SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in signal transduction from the cell surface to the nucleus. Related references: PMID:10655584 PMID:14739280 PMID:18559669
• SHP2 positively regulates the MAPK signal transduction pathway. Related references: PMID:28074573
• SHP2 dephosphorylates a range of signaling proteins, including GAB1, ARHGAP35, EGFR, ROCK2, CDC73, NEDD9/CAS-L, and mediates PDCD1-induced TCR signaling inhibition by dephosphorylating key TCR proximal molecules. Related references: PMID:28074573 PMID:18559669 PMID:26742426 PMID:19275884 PMID:32184441
• SHP2 localizes to both the cytoplasm and nucleus, facilitating its roles in signal transduction and gene regulation.
• Widely expressed, with highest levels in heart, brain, and skeletal muscle.
• Keywords include disease variant, reflecting that mutations in SHP2 are implicated in developmental disorders such as Noonan syndrome and LEOPARD syndrome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human SHP2. Consider verifying antibody specificity with endogenous SHP2 in your target cell lines.
• This antibody has been indicated for Western blotting (predicted molecular weight ~68 kDa), immunofluorescence/ICC, and ELISA. Optimize primary antibody dilutions and detection conditions for your specific experimental setup.
• The antibody cross-reacts with human, mouse, and rat SHP2, making it suitable for cross-species studies. Validate performance in the appropriate sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this high-quality anti-SHP2 rabbit monoclonal antibody to support your signal transduction research and diagnostic development. With our extensive supply chain, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your projects from concept to clinic.