Anti-SHP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q06124

Core Product Specifications and Parameters

Biological Background: SHP2 Function and Localization

• SHP2 functions as a signal transducer downstream of various receptor and cytoplasmic protein tyrosine kinases, relaying signals from the cell surface to the nucleus (PubMed:10655584, PubMed:14739280). Related references: PMID:10655584, PMID:14739280
• Positively regulates the MAPK signal transduction pathway, contributing to cell proliferation and differentiation (PubMed:28074573). Related references: PMID:28074573
• Dephosphorylates key signaling proteins including GAB1, ARHGAP35, and EGFR to fine-tune signaling outputs (PubMed:28074573). Related references: PMID:28074573
• Dephosphorylates ROCK2 at Tyr-722, stimulating its RhoA binding activity and influencing cytoskeletal dynamics (PubMed:18559669). Related references: PMID:18559669
• Dephosphorylates CDC73 and tyrosine-phosphorylated NEDD9/CAS-L, impacting transcription and cell adhesion (PubMed:26742426, PubMed:19275884). Related references: PMID:26742426, PMID:19275884
• Acts as an effector of PDCD1 (PD-1) in T-cells, contributing to inhibition of T-cell receptor signaling by dephosphorylating key proximal molecules (PubMed:32184441). Related references: PMID:32184441
• Additionally, dephosphorylates SOX9 on tyrosine residues, leading to inactivation of SOX9 and promotion of ossification (By similarity).
• Subcellular localization: Predominantly cytoplasmic but can translocate to the nucleus.
• Tissue distribution: Ubiquitously expressed with highest levels in heart, brain, and skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-460 of human SHP2 (NP_002825.3), covering the N-terminal SH2 domains and the phosphatase domain; consider that the antibody may recognize full-length SHP2 as well as N-terminal fragments in your sample system.
• For Western blot, a band around 68 kDa is expected; validate with appropriate positive and negative controls, and note that post-translational modifications or isoforms may alter migration.
• This antibody has been found to cross-react with mouse and rat SHP2; confirm sequence identity in the epitope region for your specific experimental conditions.
• For immunofluorescence/ICC, anticipate both cytoplasmic and nuclear staining; use nuclear counterstains and include negative controls (e.g., SHP2 knockout cells) to verify specificity.
• For immunoprecipitation and ELISA, titrate the antibody and optimize lysis/binding conditions for your specific sample type and target abundance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic and research reagent manufacturers, offering one-stop access to IVD raw materials spanning the full development pipeline. The Anti-SHP2 polyclonal antibody supports investigations into signal transduction, oncogenic signaling, and immune checkpoint research, and CamelBio can provide complementary validated antibody pairs, bulk ancillary reagents, and rare-target sourcing to scale your assay development. Our integrated supply network helps streamline procurement of high-quality reagents for consistent lot-to-lot performance.