Anti-SHP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P29350

Core Product Specifications and Parameters

Biological Background: SHP1 Function and Localization

• SHP1, encoded by the PTPN6 gene, is also known as hematopoietic cell protein-tyrosine phosphatase (HCP), protein-tyrosine phosphatase 1C (PTP-1C), and SH-PTP1. Alternative splicing generates isoforms with distinct tissue distributions.
• SHP1 dephosphorylates and negatively regulates several receptor tyrosine kinases (RTKs) such as EGFR, PDGFR, and FGFR, modulating their downstream signaling activities. Related references: PMID:21258366 PMID:9733788
• Upon recruitment to immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptors (e.g., LILRB1, PD-1, CD22, CLEC12A), SHP1 dephosphorylates these receptors and suppresses downstream immune activation signals. Related references: PMID:11907092 PMID:14739280 PMID:37932456
• SHP1 modulates signaling of cytokine receptors, such as the IL-4 receptor, impacting immune cell functions. Related references: PMID:9065461
• It targets cytoplasmic signaling molecules including STING1, LCK, and STAT1, thereby regulating T-cell activation and the cGAS-STING pathway. Related references: PMID:34811497 PMID:38532423
• In the nucleus, SHP1 negatively regulates transcription factors such as NFAT5 and is a key transcriptional regulator of hepatic gluconeogenesis by controlling RNA polymerase II recruitment to the PCK1 promoter together with STAT5A. Related references: PMID:37595871
• SHP1 is a phosphoprotein subject to isopeptide bond and ubiquitin conjugation, and it localizes to both the cytoplasm and nucleus.
• Isoform 1 is expressed in hematopoietic cells, while isoform 2 is found in non-hematopoietic cells, reflecting its broad roles in immune and metabolic tissues.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 100-400 of human SHP1, a region conserved across human, mouse, and rat, suggesting cross-reactivity. Validate cross-reactivity in the relevant species by western blot or immunostaining.
• For western blot (WB), a band at approximately 68 kDa is expected. Use appropriate protein extraction buffers and include positive and negative controls to confirm specificity.
• When performing immunofluorescence/immunocytochemistry (IF/ICC), consider including knockout/knockdown controls or peptide competition to verify antibody binding.
• For ELISA, the polyclonal nature may enhance signal detection, but titrate the antibody and validate with known SHP1‑expressing samples to ensure performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your diagnostic and research programs by providing reliable access to IVD raw materials. This SHP1 antibody is part of a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need immune signaling targets, rare reagents, or end‑to‑end sourcing support, CamelBio streamlines procurement to help bring your projects from concept to clinic.