Anti-SHP1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P29350

Core Product Specifications and Parameters

Biological Background: SHP1 Function and Localization

• SHP1 is a tyrosine-protein phosphatase non-receptor type 6 (PTPN6) that plays critical roles in immune signaling pathways and fundamental processes like hematopoiesis (PubMed:14739280, PubMed:29925997).
  Related references: PMID:14739280, PMID:29925997
• It negatively regulates several receptor tyrosine kinases (RTKs) including EGFR, PDGFR, and FGFR by dephosphorylating them, thereby modulating their signaling activities (PubMed:21258366, PubMed:9733788).
  Related references: PMID:21258366, PMID:9733788
• SHP1 is recruited to immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptors (e.g., LILRB1, PDCD1, CD3D, CD22, CLEC12A) to initiate their dephosphorylation and inhibit downstream signaling (PubMed:11907092, PubMed:14739280, PubMed:37932456).
  Related references: PMID:11907092, PMID:14739280, PMID:37932456
• It modulates the signaling of several cytokine receptors, including the IL-4 receptor (PubMed:9065461).
  Related references: PMID:9065461
• SHP1 targets multiple cytoplasmic signaling molecules such as STING1, LCK, and STAT1, which are involved in diverse processes like T-cell activation and cGAS-STING signaling (PubMed:34811497, PubMed:38532423).
  Related references: PMID:34811497, PMID:38532423
• Within the nucleus, SHP1 negatively regulates the activity of transcription factors (e.g., NFAT5) and controls hepatic gluconeogenesis by recruiting RNA polymerase II to the PCK1 promoter together with STAT5A (PubMed:37595871).
  Related references: PMID:37595871
• Isoform 1 is expressed in hematopoietic cells, while isoform 2 is expressed in non-hematopoietic cells, indicating tissue-specific regulation.
• Subcellularly, SHP1 localizes to both the cytoplasm and the nucleus, facilitating its diverse regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide spanning amino acids 496–595, which lies within the C-terminal region of SHP1. This region is conserved across human, mouse, and rat, consistent with the cross-reactivity profile.
• For Western blotting (WB), a band at approximately 68 kDa is expected; use appropriate positive controls from hematopoietic (isoform 1) or non-hematopoietic (isoform 2) cells depending on the target isoform.
• In immunofluorescence (IF) and immunohistochemistry (IHC), consider using tissues or cells known to express SHP1, such as immune cell lines or primary lymphoid tissues. Validate the staining pattern by comparing with known subcellular localization (cytoplasmic and nuclear).
• For ELISA applications, ensure the assay conditions are optimized for recombinant or native SHP1, and consider using a matched antibody pair for capture-detection formats.
• Since SHP1 is a key regulator in immune signaling, this antibody is particularly suitable for studies involving immune checkpoint regulation, cytokine signaling, and hematological malignancies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research labs, and academic institutes with a comprehensive one-stop sourcing platform for high-quality IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare or custom targets. For research into immune signaling, hematopoiesis, and tyrosine phosphatase biology, the anti-SHP1 antibody is just one example of the reliable tools we offer to support your assay development from concept to clinic.