Anti-SHOX Rabbit Polyclonal Antibody for WB, ELISA - O15266

Core Product Specifications and Parameters

Biological Background: SHOX Function and Localization

• SHOX (short stature homeobox protein), also known as pseudoautosomal homeobox-containing osteogenic protein, is a transcription factor encoded by the SHOX (PHOG) gene. Alternative splicing generates multiple isoforms involved in growth regulation.
• SHOX directly activates NPPB (natriuretic peptide B) transcription in osteogenic cells, a critical mechanism for controlling fundamental aspects of growth. Related references: PMID:11751690, PMID:17881654
• It preferentially binds to DNA elements containing the palindromic sequence 5'-TAATNNNATTA-3', likely functioning as a homodimeric transcription factor. Related references: PMID:11751690
• The protein contains a homeobox domain and belongs to the homeobox family of transcription factors, localizing predominantly to the nucleus.
• SHOX is highly expressed in bone marrow fibroblasts, trabecular bone cells, and the growth plate, particularly in hypertrophic chondrocytes. It is also detected in placenta, skeletal muscle, pancreas, heart, and fetal kidney. In knee cartilage, expression tends to be higher in men compared to women.
• Post-translational modifications include phosphorylation, and mutations in the SHOX gene are associated with skeletal dysplasias and short stature syndromes (e.g., Léri-Weill dyschondrosteosis, Langer mesomelic dysplasia).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 20-110) of human SHOX, which is conserved in mouse and rat, supporting the reported cross-reactivity. When working with these species, validate detection in your specific experimental system.
• For Western blot, expect a band at approximately 32 kDa. Use a molecular weight marker and optimize the gel percentage and transfer settings for proteins in this range.
• In ELISA, the recombinant protein used as the immunogen may be employed as a coating antigen to confirm antibody specificity and titer.
• Given the nuclear localization of SHOX, consider using nuclear extraction or subcellular fractionation protocols to enrich the target and improve signal in cell-based assays.
• As with any polyclonal antibody, batch-to-batch variability may occur; always include appropriate controls and titrate the antibody for optimal performance in your own assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for sourcing premium IVD raw materials, helping diagnostic manufacturers and research labs accelerate development from concept to clinic. For growth-related and skeletal disease research, we supply this anti-SHOX polyclonal antibody alongside validated antibody pairs, recombinant antigens, and ancillary reagents. Our team also supports custom sourcing and bulk procurement of rare target reagents to meet your unique assay needs.