Anti-SHOC2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UQ13

Core Product Specifications and Parameters

Biological Background: SHOC2 Function and Localization

• SHOC2 (also known as leucine-rich repeat protein SHOC-2, protein soc-2 homolog, protein sur-8 homolog) functions as a core scaffold in the SHOC2-MRAS-PP1c (SMP) holophosphatase complex, which is essential for MAPK pathway activation. Related references:
  PMID:10783161, PMID:16630891, PMID:25137548

• The SMP complex specifically dephosphorylates the inhibitory serine residues of RAF kinases, including RAF1 at Ser-259, BRAF at Ser-365, and ARAF at Ser-214, thereby relieving inhibition and stimulating their kinase activities. Related references:
  PMID:10783161, PMID:16630891, PMID:35768504

• SHOC2 enhances the dephosphorylation activity and substrate specificity of PP1c within the SMP complex, ensuring precise regulation of RAF kinase activation. Related references:
  PMID:35768504, PMID:36175670

• Acts as an inhibitor of keratinocyte differentiation by interacting with RAS protein complexes that sustain ERK activation. Related references:
  PMID:23524970

• Subcellular localization studies indicate that SHOC2 is present in both the cytoplasm and the nucleus.

• Tissue specificity: At the protein level, SHOC2 is expressed in the intermediate epidermal layers, with weaker expression in the most basal and suprabasal keratinocytes.

• The protein contains leucine-rich repeats and is subject to alternative splicing; disease-associated variants are linked to disorders such as Noonan syndrome-like disorder with loose anagen hair (NSLH1).

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1-280 of human SHOC2, covering the N-terminal region with leucine-rich repeats; this design may recognize both full-length and alternative splice isoforms.
• For western blotting, the predicted molecular weight is approximately 65 kDa; however, post-translational modifications or alternative splicing may result in additional bands.
• This antibody has been applied in WB, IF/ICC, and ELISA; optimal working dilutions should be determined empirically for each experimental system.
• Cross-reactivity is reported for human, mouse, and rat; when working with other species, confirm reactivity via sequence homology analysis and pilot experiments.
• For immunofluorescence, appropriate fixation and permeabilization methods should be validated, as SHOC2 localizes to both cytoplasmic and nuclear compartments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a complete portfolio of IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This SHOC2 polyclonal antibody is an excellent tool for studies on MAPK signaling, cancer biology, and RASopathies. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to streamline your assay development.