Anti-SHMT2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P34897

Core Product Specifications and Parameters

Biological Background: SHMT2 Function and Localization

• SHMT2 encodes mitochondrial serine hydroxymethyltransferase, also known as glycine hydroxymethyltransferase and serine methylase, a key enzyme in one-carbon metabolism.
• It catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylenetetrahydrofolate, providing one-carbon units for purine biosynthesis and other pathways. Related references: PMID:24075985, PMID:25619277, PMID:29364879
• SHMT2 contributes to de novo mitochondrial thymidylate biosynthesis, crucial for preventing uracil accumulation in mitochondrial DNA. Related references: PMID:21876188
• The enzyme supports mitochondrial translation by generating methyl donors for the modification of taurinomethyluridine at the wobble position of certain mitochondrial tRNAs. Related references: PMID:29364879, PMID:29452640
• Localized primarily to the mitochondrial matrix, inner membrane, and nucleoids, SHMT2 is also detected in the cytoplasm and nucleus, and it associates with mitochondrial DNA. Related references: PMID:18063578
• SHMT2 is subject to post-translational modifications, including acetylation and phosphorylation, and functions as a phosphoprotein and pyridoxal phosphate-dependent transferase.
• Mutations in SHMT2 have been associated with human diseases such as cardiomyopathy and intellectual disability.
• In addition to its canonical metabolic role, SHMT2 participates in the deubiquitination of target proteins, such as IFNAR1, as a component of the BRISC complex. Related references: PMID:24075985

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 265–504 of human SHMT2 (C-terminal region), which may allow detection of full-length and some annotated isoforms. Consider validating reactivity against expected splice variants if necessary.
• For Western blotting (WB), the predicted molecular weight is ~56 kDa, but actual migration may vary depending on gel conditions and post-translational modifications. Include appropriate positive and negative controls.
• Immunohistochemistry (IHC-P) may require antigen retrieval and optimization of antibody dilution. Testing on control tissues with known SHMT2 expression is recommended.
• The antibody is suitable for ELISA; consider using it to quantify SHMT2 levels in cell or tissue lysates following standard sandwich or indirect ELISA protocols.
• Cross-reactivity with mouse and rat SHMT2 is indicated, enabling translational studies. However, individual lot validation in the relevant species is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-SHMT2 reagent for one-carbon metabolism and mitochondrial research. As your one-stop sourcing partner, we supply bulk ancillary reagents and can assist in rare target raw-material procurement, streamlining your diagnostic development from concept to clinic.