Anti-SHH Rabbit Polyclonal Antibody for WB, ELISA - Q62226

Core Product Specifications and Parameters

Biological Background: Sonic hedgehog (SHH) Function and Localization

• SHH is a member of the hedgehog family, produced as a precursor protein that includes signaling and catalytic domains; the precursor is also known as ShhNC.
• The precursor undergoes autocatalytic cleavage between residues 198-199, releasing the N-terminal signaling domain (ShhN) with a C-terminal cholesterol moiety, while the C-terminal domain (ShhC) catalyzes the reaction and is subsequently degraded in the endoplasmic reticulum. Related references: PMID:7736596, PMID:7891723, PMID:8824192, PMID:21357747
• ShhN acts as a morphogen by binding to the Patched receptor (PTCH1 or PTCH2), which relieves SMO inhibition and activates the smoothened signaling pathway, essential for numerous developmental processes. Related references: PMID:11389830, PMID:31657721, PMID:14687547, PMID:29622726
• During development, SHH signaling controls ventral neural tube and floor plate specification, limb bud anterior-posterior patterning, somite formation, and axon guidance. Related references: PMID:11430830, PMID:12679031, PMID:15315762, PMID:24863049
• The full-length protein localizes to the endoplasmic reticulum and Golgi apparatus membranes; ShhN is secreted and found at the cell membrane, while ShhC remains ER-associated. Related references: PMID:21357747
• SHH is expressed in multiple embryonic tissues such as notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity, and limb mesenchyme; in adults, it is detected in lung and neural retina.
• The protein contains a signal peptide, zinc- and calcium-binding motifs, and undergoes palmitoylation; it is categorized as a glycoprotein, lipoprotein, and developmental protein.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein spanning amino acids 187-281 of mouse SHH, a region that includes the autoprocessing cleavage site. For Western blot, multiple bands may be observed corresponding to the full-length precursor (approx. 48 kDa) and processed forms such as ShhN (approx. 20 kDa) and ShhC (approx. 25-30 kDa). Validate the antibody in your specific sample and experimental context.
• For ELISA applications, this antibody can be explored as a capture or detection reagent. Since it is a polyclonal preparation, pairing with a monoclonal antibody against a different epitope may enhance assay specificity. Titration and optimization in your assay conditions are recommended.
• The antibody cross-reacts with human, mouse, and rat SHH, making it suitable for studies across these species. However, always confirm specificity via appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of raw materials, including this anti-SHH antibody for developmental biology and signaling studies. Whether you need validated antibody pairs, bulk ancillary reagents, or sourcing for rare targets, our streamlined supply chain accelerates your development from concept to clinic.