Anti-SHC1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P29353

Core Product Specifications and Parameters

Biological Background: SHC1 Function and Localization

• SHC1 encodes three main isoforms: p46Shc, p52Shc, and p66Shc, which function as signaling adapters downstream of activated growth factor receptors.
• Isoforms p46Shc and p52Shc couple receptor tyrosine kinases to the Ras/MAPK pathway by recruiting the GRB2/SOS complex, thereby propagating mitogenic signals.
• Isoform p66Shc is involved in oxidative stress responses and apoptosis, acting as a downstream target of p53 to regulate intracellular oxidant levels and cytochrome c release.
• SHC1 participates in TEK/TIE2 angiopoietin receptor signaling, promoting endothelial cell migration and sprouting angiogenesis.
• SHC1 contributes to oncostatin-M signaling through the OSMR/IL6ST receptor complex, activating Ras/Raf/MAPK pathways via JAK1-dependent recruitment to OSMR. Related references: PMID:11016927
• The protein localizes to the cytoplasm, focal adhesions, mitochondrial matrix, and mitochondria, reflecting its multifaceted roles in signal transduction.
• Widely expressed in tissues, SHC1 is present in neural stem cells but absent in mature neurons, and undergoes tyrosine phosphorylation upon activation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 300–400 within the C-terminal region of human SHC1, a segment conserved across species that likely accounts for cross-reactivity with mouse and rat.
• For western blot, the predicted molecular weight is 63 kDa; however, endogenous isoforms (p46, p52, p66) may be detected. Consider using appropriate molecular weight markers and validate in the relevant sample system.
• In IHC-P and IF/ICC, note the multi-compartment localization (cytoplasm, focal adhesions, mitochondria) and include proper controls to confirm specificity in tissue or cell models.
• For ELISA, the antibody may serve as a capture or detection reagent; optimization of coating concentrations and blocking conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials and technical services, supporting diagnostic manufacturers and researchers from concept to clinic. This SHC1 polyclonal antibody, targeting a key signaling adapter, is part of a broad portfolio of validated antibodies for signal transduction and cancer research. Beyond individual reagents, we supply antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development workflows.