Anti-SHB Rabbit Polyclonal Antibody for WB, ELISA - Q15464

Core Product Specifications and Parameters

Biological Background: SHB Function and Localization

• SHB (SH2 domain-containing adapter protein B) is a widely expressed adapter protein that links activated receptors to downstream signaling components.
• It contains an SH2 domain that enables binding to phosphotyrosine residues on activated receptors, facilitating signal complex assembly.
• SHB is involved in angiogenesis through regulation of FGFR1, VEGFR2, and PDGFR signaling pathways.
• It also mediates T-cell receptor/TCR and interleukin-2 signaling, modulates apoptosis, and promotes neuronal differentiation via basic-FGF and NGF cascades.
• In insulin-producing cells, SHB regulates IRS1 and IRS2 signaling, contributing to metabolic control.
• Subcellularly, SHB localizes predominantly to the cytoplasm and is recruited to the cell membrane upon receptor activation.
• Post-translational modifications include phosphorylation and ubiquitination (Isopeptide bond, Ubl conjugation), which may regulate its adaptor function.
• Alternative splicing produces multiple isoforms, suggesting functional diversity in different tissues or developmental stages.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 300-400 within the C-terminal region of human SHB, which includes the SH2 domain. This design may confer specificity for SHB among other SH2-containing adapters; however, validate cross-reactivity in relevant sample systems.
• The antibody is recommended for Western blot (WB) and ELISA. For WB, a band at approximately 55 kDa is expected; consider using positive controls from human, mouse, or rat cell lysates.
• Since SHB is involved in multiple signaling pathways (angiogenesis, T-cell, insulin), experimental conditions (e.g., stimulation with FGF, VEGF) may influence detection. Optimize sample preparation accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-SHB polyclonal antibody supports signal transduction and angiogenesis research applications. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD development pipeline.