Anti-SHB Rabbit Monoclonal Antibody for WB, ELISA - Q15464

Core Product Specifications and Parameters

Biological Background: SHB Function and Localization

• SHB (SH2 domain-containing adapter protein B) is an intracellular adapter protein that couples activated receptors to downstream signaling pathways.
• It plays a critical role in angiogenesis by mediating signal transduction from FGFR1, VEGFR2, and PDGFR.
• SHB is involved in T-cell antigen receptor (TCR) signaling, interleukin-2 (IL-2) signaling, apoptosis, and neuronal cell differentiation.
• The protein mediates basic-FGF and NGF-induced signaling cascades and may also regulate IRS1 and IRS2 signaling in insulin-producing cells.
• SHB localizes to the cytoplasm and cell membrane, consistent with its adapter function at the plasma membrane.
• It is widely expressed across human tissues.
• Annotated keywords include alternative splicing, angiogenesis, apoptosis, developmental protein, differentiation, isopeptide bond, phosphoprotein, SH2 domain, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 409–509) of human SHB, which encompasses the SH2 domain; this design may favor recognition of the full-length protein in its native conformation.
• For Western blotting, a band around 55 kDa is expected; use appropriate positive control lysates (e.g., Jurkat, HeLa) and validate detection in the relevant species (human, mouse, rat).
• In ELISA, consider using the full-length recombinant protein as a standard and optimizing coating and detection conditions for the specific assay format.
• To confirm biological relevance, siRNA-mediated knockdown or overexpression studies in your cell system of interest may be employed to validate antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, tailored to support diagnostic manufacturers and research laboratories in signal transduction and oncology-related projects. Our portfolio includes validated antibody pairs, recombinant monoclonal antibodies like anti-SHB, and bulk ancillary reagents for immunoassay development. If you require custom sourcing for rare targets or specialized raw materials, CamelBio’s supply chain expertise can bridge your project from concept to clinic.