Anti-SHANK2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UPX8

Core Product Specifications and Parameters

Biological Background: SHANK2 Function and Localization

• Protein identity: SHANK2, also known as SH3 and multiple ankyrin repeat domains protein 2, CortBP1, ProSAP1, is encoded by the SHANK2 gene (GeneID: 22941) in humans.
• Core function: Serves as a key adapter protein in the postsynaptic density (PSD) of excitatory synapses, interconnecting glutamate receptors (NMDA and metabotropic) with the actin cytoskeleton.
• Gene family: Member of the SHANK family of scaffold proteins, critical for synaptic structure and function; mutations are linked to autism spectrum disorders.
• Subcellular localization: Found at the apical cell membrane, cytoplasm, postsynaptic density, growth cones, and dendritic spines, reflecting its role in neuronal architecture.
• Post-translational modifications and features: Classified as a glycoprotein and phosphoprotein, with multiple ankyrin repeats and an SH3 domain that mediate protein-protein interactions.
• Tissue specificity: Isoform 3 is specifically expressed in epithelial colonic cells, while other isoforms are predominantly neuronal.
• Disease association: Implicated in autism (AUTS17) and related neurodevelopmental conditions due to synaptic dysfunction.
• Keywords highlight: Alternative splicing, SH3-binding, and presence in cell projections emphasize its regulatory versatility.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide corresponding to amino acids 500–600 of human SHANK2, the antibody may recognize epitopes within this central region. Verify specificity via knockdown or peptide competition assays.
• For western blot (WB), SHANK2 has a predicted molecular weight of ~201 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider co-staining with synaptic markers to confirm localization at the postsynaptic density.
• For ELISA, validate linearity using recombinant SHANK2 fragment covering the immunogen region.
• As cross-reactivity is confirmed for human, mouse, and rat, the antibody is suitable for comparative studies across these species; always include species-matched controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our SHANK2 antibody supports neuroscience and neurodevelopmental research with reliable antigen-specific performance. From concept to clinic, CamelBio streamlines sourcing for rare-target antibodies and custom manufacturing, helping you accelerate assay development.