Anti-SH3PXD2A Polyclonal Antibody for WB, IF/ICC, ELISA - Q5TCZ1

Core Product Specifications and Parameters

Biological Background: SH3PXD2A Function and Localization

• SH3PXD2A (SH3 and PX domain-containing protein 2A) is an adapter protein also known as TKS5, FISH, and SH3MD1. It belongs to the TKS family and contains an N-terminal PX domain and five C-terminal SH3 domains, with alternative splicing generating multiple isoforms.
• The protein is a key scaffold in invadopodia and podosome formation, facilitating extracellular matrix (ECM) degradation and promoting the invasiveness of cancer cells. It binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs), and phosphoinositides. Related references: PMID:27789576
• SH3PXD2A organizes NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and localizes ROS to invadopodia, contributing to cellular signaling and matrix remodeling.
• In association with ADAM12, SH3PXD2A mediates the neurotoxic effect of amyloid-beta peptide, linking it to neurodegenerative processes.
• The protein localizes to the cytoplasm and concentrates at cell projections, particularly podosomes, and is detected in cell junctions.
• It is predominantly expressed in cancer cell lines, with high levels found in invasive breast carcinomas and melanomas.
• Extensive phosphorylation regulates SH3PXD2A function, and its 3D structure has been characterized. The protein features coiled-coil regions and SH3 domain repeats.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 806–1105, covering the C-terminal region rich in SH3 domains. This design targets a functionally critical area, but you should consider potential isoform variability and validate recognition in your specific experimental system.
• For Western blotting, expect a band around 125 kDa; however, post-translational modifications or isoforms may produce additional bands. Use appropriate positive controls (e.g., invasive cancer cell lines) and confirm specificity with knockdown or knockout samples.
• In immunofluorescence/ICC, the antibody should label podosomes and cytoplasmic regions. Employ suitable fixation and permeabilization protocols, and co-stain with podosome markers (e.g., cortactin) to confirm localization.
• For ELISA, pair this antibody with a capture antibody if designing a sandwich assay, and optimize coating conditions using recombinant SH3PXD2A fragments.
• Due to cross-reactivity with mouse, you can validate models using mouse tissues or cell lines, but always compare expression patterns with known databases.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For research centered on invadopodia, cancer invasion, and ROS signaling, we streamline access to SH3PXD2A antibodies and related key reagents, bridging the gap from concept to clinical application. Our integrated supply chain supports diagnostic manufacturers and laboratories with reliable, research-grade tools tailored to accelerate your assay development.