Anti-SH3GL3 Polyclonal Antibody for WB, ELISA - Q99963

Core Product Specifications and Parameters

Biological Background: SH3GL3 Function and Localization

• Endophilin-A3 (SH3GL3) is a member of the endophilin family involved in clathrin-mediated endocytosis, likely recruiting proteins to highly curved membranes.
• The protein contains an N-terminal BAR domain, which senses and induces membrane curvature, and a C-terminal SH3 domain that mediates protein-protein interactions.
• SH3GL3 localizes predominantly in the cytoplasm and associates with early endosome membranes, consistent with its role in vesicular trafficking.
• Tissue expression is predominantly in brain and testis, indicating specialized functions in neuronal and reproductive tissues.
• Alternative splicing generates multiple transcript variants, as indicated by the UniProt keyword, expanding functional diversity.
• Post-translational modifications include phosphorylation, annotated as a phosphoprotein, potentially regulating its activity.
• Additional structural motifs include a coiled coil region, and the protein is classified under lipid-binding and membrane-related keywords.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein corresponding to full-length human SH3GL3 (aa 1–270), making it suitable for detecting total endogenous protein in multiple species.
• For Western blot, consider using brain or testis lysates as positive controls based on reported tissue specificity.
• Validate the antibody under your own experimental conditions, as optimal working dilutions and detection sensitivity may vary.
• For ELISA applications, the recombinant protein immunogen can serve as a reference standard for quantitative assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated polyclonal antibodies for targets like SH3GL3. Our portfolio supports endocytosis and neuroscience research with bulk antibody supplies, optimized reagent sets, and rare target sourcing. Partner with us to accelerate your assay development and production workflows.