Anti-SH3GL2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q99962

Core Product Specifications and Parameters

Biological Background: Endophilin-A1 Function and Localization

• Endophilin-A1 (SH3GL2) is encoded by the SH3GL2 gene and belongs to the endophilin family of SH3 domain-containing proteins.
• Core function in clathrin-mediated endocytosis: it facilitates ultrafast endocytosis by mediating neck formation and subsequent clathrin coat removal from regenerated vesicles.
• It plays a crucial role in BDNF-dependent dendrite outgrowth and cooperates with other proteins to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.
• Subcellularly, Endophilin-A1 localizes to the cytoplasm, membrane, early endosomes, and presynaptic regions, consistent with its role in endocytosis at neuronal synapses.
• Tissue specificity: highly expressed in the brain, particularly in the frontal cortex and at high levels in the fetal cerebellum, underscoring its importance in neuronal development and function.
• Key structural features include an SH3 domain, coiled-coil region, and lipid-binding capacity; the protein is also subject to phosphorylation, potentially regulating its activity.
• Additional annotations include its identification in proteomics studies and a reference proteome status, with a calculated molecular weight of approximately 40 kDa.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein corresponding to amino acids 1-270 of human SH3GL2, which includes the N-terminal BAR domain and SH3 domain; thus it may recognize both endophilin-A1 and potentially other isoforms or family members—consider verifying specificity in your assay.
• For Western blot (WB): The predicted molecular weight of SH3GL2 is ~40 kDa; use appropriate positive controls such as human brain lysate or mouse brain lysate to validate the band.
• In immunofluorescence (IF/ICC): Endophilin-A1 exhibits punctate staining in the cytoplasm and at the plasma membrane; co-staining with endosomal markers may help confirm localization.
• For ELISA: The polyclonal nature allows for sensitive detection; however, paired antibody testing is recommended for sandwich ELISA development.
• Cross-reactivity with mouse is specified; however, it is advisable to test with your sample species if outside human or mouse.
• The antibody is supplied as liquid, shipped on ice; upon receipt, store appropriately and avoid repeated freeze-thaw cycles to maintain activity.

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