Anti-SH3GL1 Rabbit Polyclonal Antibody for WB, ELISA - Q99961

Core Product Specifications and Parameters

Biological Background: SH3GL1 Function and Localization

• SH3GL1 encodes Endophilin-A2, also known as EEN fusion partner of MLL, Endophilin-2, SH3 domain-containing GRB2-like protein 1, and SH3 domain protein 2B.
• The protein plays a role in endocytosis, mediating the formation of highly curved membrane necks during ultrafast endocytosis and subsequently facilitating the removal of clathrin coats from regenerated vesicles.
• Subcellular localization includes the cytoplasm, early endosome membrane, and cell projections such as podosomes, linking it to membrane trafficking and cytoskeletal dynamics.
• Expression is ubiquitous, with higher levels observed in the pancreas, placenta, prostate, testis, and uterus.
• Key structural features include an SH3 domain and coiled-coil regions, enabling interactions with proline-rich motifs and membrane curvature sensing/binding.
• The protein is subject to alternative splicing and phosphorylation, and has been implicated in chromosomal rearrangements associated with leukemia (as an MLL fusion partner), highlighting its proto-oncogenic potential.
• UniProt reports a molecular weight of 41490 Da, consistent with the ~41 kDa band observed by western blot.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 219–368 of human SH3GL1, covering part of the C-terminal region including the SH3 domain. This design likely provides specificity for SH3GL1 over related endophilin family members.
• For western blot, consider using reduced samples and a 10% SDS-PAGE gel; expect a band around 41 kDa. Include positive controls such as HeLa or HEK293 cell lysates, and validate in target species (human, mouse, rat, monkey) to confirm expected cross-reactivity.
• In ELISA applications, this polyclonal antibody may be suitable as either a capture or detection reagent. Optimal coating concentrations and blocking conditions should be empirically determined.
• Because this is a polyclonal reagent, lot-to-lot consistency should be verified for critical assays. Consider including an appropriate negative control (e.g., SH3GL1 knockout cell lysate) to confirm specificity.
• If using for immunoprecipitation or other applications not listed, in-house validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials. For endocytosis and membrane trafficking studies, we offer this anti-SH3GL1 polyclonal antibody along with complementary reagents like validated antibody pairs, optimized monoclonal alternatives, and bulk ancillary reagents. Leveraging our extensive sourcing network, we also assist in procuring rare-target antibodies and custom recombinant proteins to accelerate your assay development from concept to clinic.