Anti-SH3BP2 Polyclonal Antibody for WB, ELISA - P78314

Core Product Specifications and Parameters

Biological Background: SH3BP2 Function and Localization

• SH3BP2, also known as 3BP2, is an SH3 domain-binding protein that serves as an adaptor in signal transduction pathways, linking tyrosine kinases to downstream effectors.
• The protein binds preferentially to the SH3 domains of signaling molecules such as the fes tyrosine kinase, thereby facilitating membrane recruitment and activation in hematopoietic cells.
• SH3BP2 interacts with phosphatidylinositols in a phosphorylation-dependent manner, tethering signaling complexes to the cytoplasmic membrane.
• It is widely expressed across tissues, including lung, liver, skeletal muscle, kidney, and pancreas, suggesting a broad functional role in cellular communication.
• The protein features an SH2 domain that mediates phosphotyrosine interactions, along with a proline-rich region recognized by SH3 domains, classifying it as a dual adaptor molecule.
• Alternative splicing yields distinct isoforms, potentially diversifying its regulatory functions in different cell types.
• Clinically, mutations in SH3BP2 are associated with cherubism, a rare bone disorder, underscoring its importance in bone homeostasis and inflammatory signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment covering amino acids 392–561, which lies within the C‑terminal region; this may facilitate detection of full‑length SH3BP2 and some splice variants.
• As a rabbit polyclonal antibody, it typically recognizes multiple epitopes, potentially offering a strong signal in Western blot when used at appropriate dilutions on human or mouse cell lysates.
• For ELISA, consider coating with recombinant SH3BP2 or using capture/detection pair formats; cross‑reactivity with mouse protein suggests utility in rodent model studies.
• Validate specificity in the relevant biological system by including appropriate controls (e.g., blocking peptide, knockout lysates) and optimize primary antibody concentration empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for manufacturers and laboratories, offering this anti‑SH3BP2 polyclonal antibody alongside a full portfolio of validated antibodies, antigens, and ancillary reagents. Whether you need antibody pairs, monoclonal alternatives, or bulk supplies for signal transduction research, our integrated supply chain accommodates projects from early development to clinical application. Let CamelBio be your bridge to reliable, high‑quality materials for diagnostic innovation.