Anti-SH3BGRL Polyclonal Antibody for WB, IF/ICC, ELISA - O75368

Core Product Specifications and Parameters

Biological Background: SH3BGRL Function and Localization

• SH3BGRL (SH3 domain-binding glutamic acid-rich-like protein) is an adapter protein encoded by the SH3BGRL gene, also known as SH3 domain-binding glutamic acid-rich-like protein 1.
• It functions as a molecular bridge between proteins and mRNAs, and additionally acts as a ubiquitin ligase-substrate adapter, facilitating ubiquitination processes. Related references: PMID:34331014, PMID:34870550
• The protein associates with translating cytoplasmic ribosomes and may promote the expression of specific mRNAs. Related references: PMID:34331014, PMID:34870550
• Subcellular localization is primarily cytosolic, with presence also at the cell membrane.
• It is ubiquitously expressed across human tissues.
• The protein contains an SH3-binding motif, and a 3D structure has been determined; it belongs to the reference proteome.
• Predicted molecular weight is approximately 12.8 kDa, consistent with the observed 13 kDa band.

Experimental Guidance and Technical Tips

• For Western blot, expect a band around 13 kDa; validate the antibody with appropriate positive controls (e.g., HeLa, HEK293 cell lysates) and loading controls.
• In immunofluorescence/ICC, permeabilization may be required to detect cytoplasmic/cytosolic localization; membrane staining could also be observed.
• For ELISA, the recombinant immunogen (amino acids 1-114) can serve as a reliable positive control.
• Validate cross-reactivity in your specific experimental model (human, mouse, or rat) using the appropriate sample system.
• For ubiquitination-related studies, consider co-immunoprecipitation or ubiquitination assays to investigate adapter-substrate interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for diagnostic manufacturers and research laboratories, providing this SH3BGRL polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether your work focuses on adapter proteins, translation regulation, or ubiquitination, our supply chain streamlines access to rare target raw materials and technical support, bridging the gap from concept to clinic.