Anti-SH2D1A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O60880

Core Product Specifications and Parameters

Biological Background: SH2D1A Function and Localization

• SH2 domain-containing protein 1A (SH2D1A), also known as signaling lymphocytic activation molecule (SLAM)-associated protein (SAP), is a cytoplasmic adapter protein critical for immune cell signaling (O60880).
• SH2D1A regulates receptors of the SLAM family, including SLAMF1, CD244, LY9, CD84, SLAMF6, and SLAMF7, acting as a key modulator of lymphocyte activation.
• Early studies proposed that SH2D1A blocks the association of SLAMF1 with inhibitory effectors SHIP1 and SHP-2, preventing negative signaling (PubMed:11806999). Related references: PMID:11806999
• Subsequent work revealed that SH2D1A instead recruits the tyrosine kinase FYN to phosphorylate and activate SLAMF1, initiating downstream signaling (PubMed:12458214). Related references: PMID:12458214
• SH2D1A positively regulates natural killer (NK) cell cytotoxicity by enhancing conjugate formation with target cells through receptors such as CD244, CD84, CD48, SLAMF6, LY9, and SLAMF7.
• It may also modulate signaling of neurotrophin receptors NTRK1, NTRK2, and NTRK3, suggesting roles beyond the immune system.
• SH2D1A is predominantly cytoplasmic and exhibits tissue-specific expression, with high levels in thymus and lung, lower in spleen and liver, and is present in T-lymphocytes; expression is reduced in rheumatoid arthritis.
• Mutations in SH2D1A are linked to X-linked lymphoproliferative disease type 1 (XLP1 or Duncan disease), an often-fatal immunodeficiency characterized by abnormal responses to Epstein-Barr virus infection.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against the full-length human SH2D1A protein (aa 1-128); it may detect endogenous levels of SH2D1A in samples from human and mouse.
• For Western blot (WB), the predicted molecular weight is approximately 14 kDa; include positive and negative controls to confirm specificity.
• In immunofluorescence (IF) / immunocytochemistry (ICC), optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) conditions for your cell type.
• For ELISA applications, this antibody can be used as a capture or detection reagent; validate with appropriate standards and pairing antibodies for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-SH2D1A antibody supports studies in immune signaling, NK cell biology, and X-linked lymphoproliferative diseases. Contact our team to explore customized sourcing solutions for rare targets and technical consultations from concept to clinic.